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Overexpression of phosphoinositide-3-kinase class II alpha enhances mesenchymal stem cell survival in infarcted myocardium.

DC FieldValueLanguage
dc.contributor.author김일권-
dc.contributor.author송병욱-
dc.contributor.author이세연-
dc.contributor.author임소연-
dc.contributor.author장양수-
dc.contributor.author장우철-
dc.contributor.author차민지-
dc.contributor.author최은미-
dc.contributor.author최은주-
dc.contributor.author함온주-
dc.contributor.author황기철-
dc.date.accessioned2015-04-23T17:28:31Z-
dc.date.available2015-04-23T17:28:31Z-
dc.date.issued2010-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/102534-
dc.description.abstractThe efficacy of mesenchymal stem cell (MSC) therapy for myocardial regeneration is limited by the poor survival of stem cells after transplantation into the infarcted heart. To improve the cell survival of MSCs in the infarcted heart, MSCs were genetically engineered to overexpress phosphoinositide-3-kinase class II alpha (PI3K-C2α). PI3K-C2α overexpression increased PI3K expression and the cell viability of MSCs. Furthermore, levels of survival-related phosphorylation were elevated in PI3K-C2α-MSCs. But, the level of apoptotic proteins downregulated and the number of PI-positive cells decreased in PI3K-C2α-MSCs compared to hypoxic MSCs. Nine rats per group had 1×10(6) cells (20 μl PBS) transplanted after myocardial infarction. One week after transplantation, infarct size and area of fibrosis were reduced in the PI3K-C2α-MSC-transplanted group. The number of TUNEL positive cells declined, while the mean microvessel count per field was higher in the PI3K-C2α-MSC group than the MSC-injected group. Heart function was improved in the PI3K-C2α-MSCs group as assessed using a Millar catheter at 3weeks after transplantation. These findings suggest that overexpression of PI3K-C2α in MSCs can assist cell survival and enhance myocardial regeneration.-
dc.description.statementOfResponsibilityopen-
dc.format.extent272~279-
dc.relation.isPartOfBiochemical and Biophysical Research Communications-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis/genetics-
dc.subject.MESHCell Survival/genetics-
dc.subject.MESHClass II Phosphatidylinositol 3-Kinases/genetics*-
dc.subject.MESHGenetic Engineering-
dc.subject.MESHHeart/physiology*-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stem Cell Transplantation*-
dc.subject.MESHMesenchymal Stromal Cells/enzymology*-
dc.subject.MESHMyocardial Infarction/enzymology-
dc.subject.MESHMyocardial Infarction/pathology-
dc.subject.MESHMyocardial Infarction/therapy*-
dc.subject.MESHMyocardium/enzymology*-
dc.subject.MESHMyocardium/pathology-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHRegeneration/genetics*-
dc.titleOverexpression of phosphoinositide-3-kinase class II alpha enhances mesenchymal stem cell survival in infarcted myocardium.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorLucy Youngmin Eun-
dc.contributor.googleauthorByeong-Wook Song-
dc.contributor.googleauthorMin-Ji Cha-
dc.contributor.googleauthorHeesang Song-
dc.contributor.googleauthorIl-Kwon Kim-
dc.contributor.googleauthorEunmi Choi-
dc.contributor.googleauthorWoochul Chang-
dc.contributor.googleauthorSoyeon Lim-
dc.contributor.googleauthorEun Ju Choi-
dc.contributor.googleauthorOnju Ham-
dc.contributor.googleauthorSe-Yeon Lee-
dc.contributor.googleauthorKi Hyun Byun-
dc.contributor.googleauthorYangsoo Jang-
dc.contributor.googleauthorKi-Chul Hwang-
dc.identifier.doi10.1016/j.bbrc.2010.10.013-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02026-
dc.contributor.localIdA02880-
dc.contributor.localIdA03448-
dc.contributor.localIdA03452-
dc.contributor.localIdA03995-
dc.contributor.localIdA04160-
dc.contributor.localIdA04336-
dc.contributor.localIdA04456-
dc.contributor.localIdA04151-
dc.contributor.localIdA00848-
dc.contributor.localIdA03373-1-
dc.relation.journalcodeJ00281-
dc.identifier.pmid20937252-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X10018644-
dc.subject.keywordCardiac regeneration-
dc.subject.keywordLentivirus-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordPhosphoinositide-3-kinase class II alpha-
dc.subject.keywordSurvival-
dc.contributor.alternativeNameKim, Il Kwon-
dc.contributor.alternativeNameSong, Byeong Wook-
dc.contributor.alternativeNameLee, Se Yeon-
dc.contributor.alternativeNameLim, So Yeon-
dc.contributor.alternativeNameJang, Yang Soo-
dc.contributor.alternativeNameChang, Woo Chul-
dc.contributor.alternativeNameCha, Min Ji-
dc.contributor.alternativeNameChoi, Eun Mi-
dc.contributor.alternativeNameChoi, Eun Ju-
dc.contributor.alternativeNameHam, On Ju-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.affiliatedAuthorSong, Byeong Wook-
dc.contributor.affiliatedAuthorLee, Se Yeon-
dc.contributor.affiliatedAuthorJang, Yang Soo-
dc.contributor.affiliatedAuthorChang, Woo Chul-
dc.contributor.affiliatedAuthorCha, Min Ji-
dc.contributor.affiliatedAuthorChoi, Eun Ju-
dc.contributor.affiliatedAuthorHam, On Ju-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorChoi, Eun Mi-
dc.contributor.affiliatedAuthorKim, Il Kwon-
dc.contributor.affiliatedAuthorLim, So Yeon-
dc.citation.volume402-
dc.citation.number2-
dc.citation.startPage272-
dc.citation.endPage279-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, Vol.402(2) : 272-279, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers

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