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Immunohistochemical localization of cytokeratins in the junctional region of ectoderm and endoderm.

Authors
 AKIHIRO HOSOYA  ;  SUNGWOOK KWAK  ;  EUN-JUNG KIM  ;  DECLAN PATRICK LUNNY  ;  E. BIRGITTE LANE  ;  SUNG-WON CHO  ;  HAN-SUNG JUNG 
Citation
 Anatomical Record - Advances in Integrative Anatomy and Evolutionary Biology, Vol.293(11) : 1864-1872, 2010 
Journal Title
 Anatomical Record - Advances in Integrative Anatomy and Evolutionary Biology 
ISSN
 1932-8464 
Issue Date
2010
Abstract
Although tridermic species have two junctional regions of ectoderm and endoderm between their epidermis and digestive tract, we actually know little about these particular boundaries. Cytokeratins are the major intermediate filaments of epithelial cells and show a high degree of tissue specificity. Therefore, to characterize the epithelial cells in the junctional region of ectoderm and endoderm, we immunohistochemically examined the localization of cytokeratins 5, 7/17, 14, 18, Sox17, and alpha-fetoprotein (AFP) in the oropharyngeal and anorectal regions during the mouse gastrulation process. At embryonic day (E) 9.5, cytokeratins 5, 7/17, 14, and 18 were detected in all epithelial cells of the oropharyngeal region. At E12.5, cytokeratin 5-positive cells were not observed in the middle area of the oral cavity; however, the immunoreactivity was strong in the anterior and posterior areas. The immunoreaction of cytokeratins 18 was seen only in the middle and posterior areas of the oral mucosa. Cytokeratins 7/17 and 14 were localized in all areas of the oropharyngeal region. Sox17 and AFP, which are endodermal markers, were detected in the middle and posterior areas of the oral mucosa, but not in the anterior area. Moreover, this same localization pattern of cytokeratins also existed in the anorectal region of the E12.5 embryo, suggesting that the localization of cytokeratins and endodermal markers might give an implication for the boundary between ectoderm and endoderm. These results also suggest that these cytokeratins are useful molecules for monitoring the epithelial cell differentiation in the junctional region of the germ layers.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102254
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Research Center for Orofacial Hard Tissue Regeneration (구강악안면경조직재생연구센터)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
Yonsei Authors
곽성욱(Kwak, Sung Wook) ; 김은정(Kim, Eun Jung) ; 정한성(Jung, Han Sung)
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