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Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells

 Uk Yeol Moon  ;  Jung Ho Bae  ;  Chang-Hoon Kim  ;  Hyun Jik Kim  ;  Ju Wan Kang  ;  Joo-Heon Yoon 
 JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.111(2) : 476-487, 2010 
Journal Title
Issue Date
Anti-Infective Agents ; Cell Line ; Epithelial Cells/metabolism* ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Gene Expression/drug effects* ; Humans ; Muramidase/drug effects ; Muramidase/genetics* ; Proto-Oncogene Proteins c-myb/metabolism* ; Respiratory System/cytology* ; Signal Transduction/drug effects ; Tetradecanoylphorbol Acetate/pharmacology* ; Transcription Factors
Lysozyme is a major component of airway epithelial secretions, acts as cationic anti-microbial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen-activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that overexpression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase-c-Myb signaling pathways in NCI-H292 cells
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Ju Wan(강주완)
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Moon, Uk Yeol(문욱열)
Bae, Jung Ho(배정호)
Yoon, Joo Heon(윤주헌)
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