Cited 6 times in
Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 강주완 | - |
dc.contributor.author | 김창훈 | - |
dc.contributor.author | 문욱열 | - |
dc.contributor.author | 배정호 | - |
dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2015-04-23T17:14:18Z | - |
dc.date.available | 2015-04-23T17:14:18Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102083 | - |
dc.description.abstract | Lysozyme is a major component of airway epithelial secretions, acts as cationic anti-microbial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen-activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that overexpression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase-c-Myb signaling pathways in NCI-H292 cells | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 476~487 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Infective Agents | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism | - |
dc.subject.MESH | Gene Expression/drug effects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Muramidase/drug effects | - |
dc.subject.MESH | Muramidase/genetics* | - |
dc.subject.MESH | Proto-Oncogene Proteins c-myb/metabolism* | - |
dc.subject.MESH | Respiratory System/cytology* | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Tetradecanoylphorbol Acetate/pharmacology* | - |
dc.subject.MESH | Transcription Factors | - |
dc.title | Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Uk Yeol Moon | - |
dc.contributor.googleauthor | Jung Ho Bae | - |
dc.contributor.googleauthor | Chang-Hoon Kim | - |
dc.contributor.googleauthor | Hyun Jik Kim | - |
dc.contributor.googleauthor | Ju Wan Kang | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1002/jcb.22730 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01050 | - |
dc.contributor.localId | A00081 | - |
dc.contributor.localId | A01371 | - |
dc.contributor.localId | A01806 | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J01303 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.identifier.pmid | 20524209 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jcb.22730/abstract | - |
dc.contributor.alternativeName | Kang, Ju Wan | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Moon, Uk Yeol | - |
dc.contributor.alternativeName | Bae, Jung Ho | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.contributor.affiliatedAuthor | Kang, Ju Wan | - |
dc.contributor.affiliatedAuthor | Moon, Uk Yeol | - |
dc.contributor.affiliatedAuthor | Bae, Jung Ho | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.citation.volume | 111 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 476 | - |
dc.citation.endPage | 487 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.111(2) : 476-487, 2010 | - |
dc.identifier.rimsid | 52713 | - |
dc.type.rims | ART | - |
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