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Epicatechin gallate suppresses oxidative stress-induced MUC5AC overexpression by interaction with epidermal growth factor receptor.

 Hyun Jik Kim  ;  Ji-Hwan Ryu  ;  Chang-Hoon Kim  ;  Jun Wan Lim  ;  Uk Yeol Moon  ;  Gyu Ho Lee  ;  Jeung-Gweon Lee  ;  Seung Joon Baek  ;  Joo-Heon Yoon 
 American Journal of Respiratory Cell and Molecular Biology, Vol.43(3) : 349-357, 2010 
Journal Title
 American Journal of Respiratory Cell and Molecular Biology 
Issue Date
Antioxidants/pharmacology* ; Blotting, Western ; Catechin/analogs & derivatives* ; Catechin/pharmacology ; Cell Proliferation ; Cells, Cultured ; Epithelial Cells/metabolism ; Fluorescent Antibody Technique ; Humans ; Hydrogen Peroxide/pharmacology ; Mucin 5AC/genetics ; Mucin 5AC/metabolism* ; Nasal Mucosa/cytology ; Nasal Mucosa/drug effects* ; Nasal Mucosa/metabolism ; Oxidants/pharmacology ; Oxidative Stress/drug effects* ; Phosphorylation/drug effects ; RNA, Messenger/genetics ; Reactive Oxygen Species/metabolism ; Receptor, Epidermal Growth Factor/genetics ; Receptor, Epidermal Growth Factor/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/drug effects
epicatechin gallate ; epidermal growth factor receptor ; mucus hypersecretion ; oxidative stress
The goal of this study was to investigate the effect of epicatechin gallate (ECG), a component of green tea polyphenols, on the signal pathway for oxidative stress-induced intracellular reactive oxygen species (ROS) generation and MUC5AC overexpression in normal human nasal epithelial (NHNE) cells. Passage-2 NHNE cells were used, and ECG was administered before stimulation with exogenous hydrogen peroxide (H(2)O(2)). MUC5AC gene and protein levels were measured by real-time PCR and dot blot analysis. Western blot analysis and immunocytofluorescence study were performed for detecting the activity of epidermal growth factor receptor (EGFR). Exogenous H(2)O(2) increases intracellular ROS generation, leading to the overexpression of MUC5AC. The phosphorylation and internalization of EGFR were associated with this ROS generation. ECG decreased the phosphorylation and internalization of EGFR at the cell surface of NHNE cells, resulting in the attenuation of exogenous H(2)O(2)-induced intracellular ROS generation and MUC5AC overexpression. ECG may be a therapeutic material against oxidative stress-induced ROS generation and mucus hypersecretion in airways.
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5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Moon, Uk Yeol(문욱열)
Ryu, Ji Hwan(유지환)
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
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