1 208

Cited 2 times in

Functional study of mucus secretion of the eustachian tube in Guinea pigs

Authors
 Hyun Seung Choi  ;  Won Sun Yang  ;  Sang Cheol Kim  ;  Won Il Lee  ;  Hyun Jae Lee  ;  Jae Young Choi 
Citation
 OTOLOGY & NEUROTOLOGY, Vol.31(5) : 817-822, 2010 
Journal Title
 OTOLOGY & NEUROTOLOGY 
ISSN
 1531-7129 
Issue Date
2010
MeSH
Adrenergic alpha-Agonists/pharmacology ; Adrenergic beta-Agonists/pharmacology ; Animals ; Calcium/agonists ; Calcium/physiology ; Carbachol/pharmacology ; Colforsin/pharmacology ; Computer Systems ; Cyclic AMP/metabolism ; Eustachian Tube/anatomy & histology ; Eustachian Tube/secretion* ; Exocrine Glands/secretion ; Guinea Pigs ; In Vitro Techniques ; Isoproterenol/pharmacology ; Kinetics ; Male ; Mucus/secretion* ; Nicotinic Agonists/pharmacology ; Phenylephrine/pharmacology ; Receptors, Adrenergic, alpha/drug effects ; Receptors, Adrenergic, alpha/physiology ; Receptors, Adrenergic, beta/drug effects ; Receptors, Adrenergic, beta/physiology ; Substance P/pharmacology
Keywords
Eustachian tube ; Submucous gland ; Mucus secretion ; Bubble study
Abstract
OBJECTIVES: Our aim was to verify neural regulation of submucous gland mucus secretions in the Eustachian tubes of guinea pigs. STUDY DESIGN: Prospective animal study. METHODS: Eustachian tubes harvested from 12 guinea pigs were used for this study. For real-time resolution of pure glandular secretion, we used a modified method of single-gland optical measurement. Secretory monitoring was undertaken after each preparation with phenylephrine, isoproterenol, forskolin, and substance P. To confirm the viability of each tissue, we examined glandular secretion after treatment with carbachol. Secretory effects of each agonist were evaluated by comparing with basal secretion using a Student's t test (p < 0.01). RESULTS: The Ca-elevating agonists carbachol and substance P showed greater effects on submucous gland secretions of the Eustachian tube than the cyclic adenosine monophosphate (cAMP)-elevating agonists forskolin and isoproterenol. However, phenylephrine, although it belongs to the Ca-elevating agonist group, did not show any significant secretory effect. CONCLUSION: The optical measurement method used in this study had the merit of real-time resolution of submucous glandular secretion. Submucous glandular secretion in the Eustachian tube was regulated by both Ca- and cAMP-elevating agonists, and Ca-elevating agonists seemed to be more potent than cAMP-elevating agonists except phenylephrine. Our results suggest that not only the autonomic nerve system but also the neuropeptides such as substance P are closely related to glandular secretion in the Eustachian tube, and beta-adrenergic receptors seem to be more related to submucous glandular secretion of the Eustachian tube in guinea pig than alpha-adrenergic receptors.
Full Text
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00129492-201007000-00019&LSLINK=80&D=ovft
DOI
10.1097/MAO.0b013e3181d35e69
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Sang Cheol(김상철)
Yang, Won Sun(양원선)
Lee, Hyun Jae(이현재)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101995
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse