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Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma.

 Young Woo Cheon  ;  Won Jai Lee  ;  Hyun Sook Baek  ;  Young Dae Lee  ;  Jong-Chul Park  ;  Young Hwan Park  ;  Chang Seok Ki  ;  Kie-Hyung Chung  ;  Dong Kyun Rah 
 Artificial Organs, Vol.34(5) : 384-392, 2010 
Journal Title
 Artificial Organs 
Issue Date
Animals ; Argon/chemistry ; Bombyx/chemistry ; Cartilage, Articular/cytology ; Cell Adhesion ; Cell Proliferation ; Cells, Cultured ; Chondrocytes/cytology* ; Chondrocytes/metabolism ; Fibroins/chemistry* ; Fibroins/isolation & purification ; Glycosaminoglycans/metabolism ; Humans ; Keratins/chemistry* ; Microwaves ; Porosity ; Surface Properties ; Tissue Scaffolds* ; Water/chemistry ; Wool/chemistry*
Cartilage tissue engineering ; Chondrocytes ; Microwave‐induced argon plasma ; Nanofibrous silk fibroin ; Scaffold
Silk fibroin (SF) is a natural, degradable, fibrous protein that is biocompatible, is easily processed, and possesses unique mechanical properties. Another natural material, wool keratose (WK), is a soluble derivative of wool keratin, containing amino acid sequences that induce cell adhesion. Here, we blended SF and WK to improve the poor electrospinability of WK and increase the adhesiveness of SF. We hypothesized that microwave-induced argon plasma treatment would improve chondrogenic cell growth and cartilage-specific extracellular matrix formation on a three-dimensional SF/WK scaffold. After argon plasma treatment, static water contact angle measurement revealed increased hydrophilicity of the SF/WK scaffold, and scanning electron microscopy showed that treated SF/WK scaffolds had deeper and more cylindrical pores than nontreated scaffolds. Attachment and proliferation of neonatal human knee articular chondrocytes on treated SF/WK scaffolds increased significantly, followed by increased glycosaminoglycan synthesis. Our results suggest that microwave-induced, plasma-treated SF/WK scaffolds have potential in cartilage tissue engineering.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Baek, Hyun Sook(백현숙)
Lee, Young Dae(이영대)
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
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