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Usefulness of melanoma antigen (MAGE) gene analysis in tissue samples from percutaneous needle aspiration biopsy of suspected lung cancer lesions

Authors
 Hana Kim  ;  Sang Jin Kim  ;  Sun-Hwa Lee  ;  Hye Soon Seong  ;  Kyung-Ok Lee  ;  Chang-Ho Jeon  ;  Yoo Jin Hong  ;  Sang Min Lee  ;  Tae Hoon Kim 
Citation
 LUNG CANCER, Vol.69(3) : 284-288, 2010 
Journal Title
 LUNG CANCER 
ISSN
 0169-5002 
Issue Date
2010
MeSH
Adenocarcinoma/diagnosis ; Adenocarcinoma/genetics* ; Adenocarcinoma/pathology ; Adenocarcinoma/physiopathology ; Adult ; Aged ; Antigens, Neoplasm/genetics ; Antigens, Neoplasm/immunology ; Antigens, Neoplasm/metabolism* ; Biopsy, Needle ; Early Detection of Cancer ; Female ; Humans ; Lung/metabolism* ; Lung/pathology ; Lung Neoplasms/diagnosis ; Lung Neoplasms/genetics* ; Lung Neoplasms/pathology ; Lung Neoplasms/physiopathology ; Male ; Melanoma/metabolism ; Middle Aged ; Polymerase Chain Reaction ; Predictive Value of Tests ; Reproducibility of Results ; Sensitivity and Specificity ; Tomography, X-Ray Computed
Keywords
Melanoma antigen (MAGE) genes ; Fine needle aspiration biopsy ; Reverse transcription-nested polymerase chain reaction (RT-nested PCR) ; Lung cancer ; Sensitivity and specificity ; Detection method
Abstract
BACKGROUND: As mortality from lung cancer is still very high, early detection prior to metastasis is important in clinical settings. We prospectively evaluated the clinical usefulness of a reverse transcription-nested polymerase chain reaction (RT-nested PCR) using melanoma antigen (MAGE) A1-6 genes with tissue samples obtained from the percutaneous needle aspiration (PCNA) biopsies used in the diagnosis of lung cancer. METHODS: We enrolled 53 patients with suspected lung cancer based on CT scan (M:F, 39:14; mean age 61 years). A PCNA biopsy was performed twice and lung cancer was diagnosed by a pathological examination. The MAGE genes were analyzed using RT-nested PCR from tissue samples obtained from the PCNA biopsy of the lesion. We compared the results from the RT-nested PCR and the pathologic diagnosis. We also analyzed the sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV). RESULTS: Of the 53 patients, 39 were diagnosed with lung cancer. Six patients had tuberculosis and 8 were confirmed with chronic inflammation or benign lesion. Based on the RT-nested PCR examination, 41 of 53 patients were positive for the MAGE gene: 34 of 39 patients had lung cancer; 5 of 6 patients had tuberculosis; and 2 of 8 patients had chronic inflammation or benign lesion. The sensitivity, specificity, accuracy, PPV and NPV were 83%, 58%, 77%, 87% and 55%, respectively. CONCLUSION: MAGE gene analysis by RT-nested PCR may be a useful method for the diagnosis of lung cancer, but it is still limited in patients with tuberculosis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0169500209006394
DOI
10.1016/j.lungcan.2009.12.003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sang Jin(김상진)
Kim, Tae Hoon(김태훈) ORCID logo https://orcid.org/0000-0003-3598-2529
Lee, Sang Min(이상민)
Hong, Yoo Jin(홍유진) ORCID logo https://orcid.org/0000-0002-7276-0944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101473
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