Cited 11 times in
Usefulness of melanoma antigen (MAGE) gene analysis in tissue samples from percutaneous needle aspiration biopsy of suspected lung cancer lesions
DC Field | Value | Language |
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dc.contributor.author | 김상진 | - |
dc.contributor.author | 김태훈 | - |
dc.contributor.author | 이상민 | - |
dc.contributor.author | 홍유진 | - |
dc.date.accessioned | 2015-04-23T16:55:01Z | - |
dc.date.available | 2015-04-23T16:55:01Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0169-5002 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101473 | - |
dc.description.abstract | BACKGROUND: As mortality from lung cancer is still very high, early detection prior to metastasis is important in clinical settings. We prospectively evaluated the clinical usefulness of a reverse transcription-nested polymerase chain reaction (RT-nested PCR) using melanoma antigen (MAGE) A1-6 genes with tissue samples obtained from the percutaneous needle aspiration (PCNA) biopsies used in the diagnosis of lung cancer. METHODS: We enrolled 53 patients with suspected lung cancer based on CT scan (M:F, 39:14; mean age 61 years). A PCNA biopsy was performed twice and lung cancer was diagnosed by a pathological examination. The MAGE genes were analyzed using RT-nested PCR from tissue samples obtained from the PCNA biopsy of the lesion. We compared the results from the RT-nested PCR and the pathologic diagnosis. We also analyzed the sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV). RESULTS: Of the 53 patients, 39 were diagnosed with lung cancer. Six patients had tuberculosis and 8 were confirmed with chronic inflammation or benign lesion. Based on the RT-nested PCR examination, 41 of 53 patients were positive for the MAGE gene: 34 of 39 patients had lung cancer; 5 of 6 patients had tuberculosis; and 2 of 8 patients had chronic inflammation or benign lesion. The sensitivity, specificity, accuracy, PPV and NPV were 83%, 58%, 77%, 87% and 55%, respectively. CONCLUSION: MAGE gene analysis by RT-nested PCR may be a useful method for the diagnosis of lung cancer, but it is still limited in patients with tuberculosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 284~288 | - |
dc.relation.isPartOf | LUNG CANCER | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenocarcinoma/diagnosis | - |
dc.subject.MESH | Adenocarcinoma/genetics* | - |
dc.subject.MESH | Adenocarcinoma/pathology | - |
dc.subject.MESH | Adenocarcinoma/physiopathology | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Antigens, Neoplasm/genetics | - |
dc.subject.MESH | Antigens, Neoplasm/immunology | - |
dc.subject.MESH | Antigens, Neoplasm/metabolism* | - |
dc.subject.MESH | Biopsy, Needle | - |
dc.subject.MESH | Early Detection of Cancer | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lung/metabolism* | - |
dc.subject.MESH | Lung/pathology | - |
dc.subject.MESH | Lung Neoplasms/diagnosis | - |
dc.subject.MESH | Lung Neoplasms/genetics* | - |
dc.subject.MESH | Lung Neoplasms/pathology | - |
dc.subject.MESH | Lung Neoplasms/physiopathology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Melanoma/metabolism | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Predictive Value of Tests | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Tomography, X-Ray Computed | - |
dc.title | Usefulness of melanoma antigen (MAGE) gene analysis in tissue samples from percutaneous needle aspiration biopsy of suspected lung cancer lesions | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiology (영상의학) | - |
dc.contributor.googleauthor | Hana Kim | - |
dc.contributor.googleauthor | Sang Jin Kim | - |
dc.contributor.googleauthor | Sun-Hwa Lee | - |
dc.contributor.googleauthor | Hye Soon Seong | - |
dc.contributor.googleauthor | Kyung-Ok Lee | - |
dc.contributor.googleauthor | Chang-Ho Jeon | - |
dc.contributor.googleauthor | Yoo Jin Hong | - |
dc.contributor.googleauthor | Sang Min Lee | - |
dc.contributor.googleauthor | Tae Hoon Kim | - |
dc.identifier.doi | 10.1016/j.lungcan.2009.12.003 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01086 | - |
dc.contributor.localId | A02815 | - |
dc.contributor.localId | A00528 | - |
dc.contributor.localId | A04422 | - |
dc.relation.journalcode | J02174 | - |
dc.identifier.eissn | 1872-8332 | - |
dc.identifier.pmid | 20064673 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0169500209006394 | - |
dc.subject.keyword | Melanoma antigen (MAGE) genes | - |
dc.subject.keyword | Fine needle aspiration biopsy | - |
dc.subject.keyword | Reverse transcription-nested polymerase chain reaction (RT-nested PCR) | - |
dc.subject.keyword | Lung cancer | - |
dc.subject.keyword | Sensitivity and specificity | - |
dc.subject.keyword | Detection method | - |
dc.contributor.alternativeName | Kim, Sang Jin | - |
dc.contributor.alternativeName | Kim, Tae Hoon | - |
dc.contributor.alternativeName | Lee, Sang Min | - |
dc.contributor.alternativeName | Hong, Yoo Jin | - |
dc.contributor.affiliatedAuthor | Kim, Tae Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Sang Min | - |
dc.contributor.affiliatedAuthor | Kim, Sang Jin | - |
dc.contributor.affiliatedAuthor | Hong, Yoo Jin | - |
dc.citation.volume | 69 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 284 | - |
dc.citation.endPage | 288 | - |
dc.identifier.bibliographicCitation | LUNG CANCER, Vol.69(3) : 284-288, 2010 | - |
dc.identifier.rimsid | 47755 | - |
dc.type.rims | ART | - |
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