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Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 in a beta-tricalcium phosphate carrier into one-wall intrabony defects in dogs.

Authors
 Jung-Seok Lee  ;  Ulf M. E. Wikesjö  ;  Ui-Won Jung  ;  Seong-Ho Choi  ;  Susanne Pippig  ;  Michael Siedler  ;  Chong-Kwan Kim 
Citation
 JOURNAL OF CLINICAL PERIODONTOLOGY, Vol.37(4) : 382-389, 2010 
Journal Title
 JOURNAL OF CLINICAL PERIODONTOLOGY 
ISSN
 0303-6979 
Issue Date
2010
MeSH
Absorbable Implants* ; Alveolar Bone Loss/therapy ; Alveolar Process/drug effects ; Alveolar Process/physiology ; Alveolar Process/surgery ; Animals ; Biocompatible Materials/administration & dosage ; Bone Regeneration/drug effects* ; Bone Regeneration/physiology ; Bone Substitutes/administration & dosage ; Calcium Phosphates/administration & dosage ; Dental Cementum/drug effects ; Dental Cementum/physiology ; Disease Models, Animal ; Dogs ; Drug Carriers/administration & dosage ; Growth Differentiation Factor 5/administration & dosage ; Growth Differentiation Factor 5/physiology* ; Humans ; Male ; Mandible/drug effects ; Mandible/surgery ; Osseointegration/drug effects ; Osseointegration/physiology ; Periodontal Attachment Loss/drug therapy* ; Periodontal Ligament/drug effects ; Periodontal Ligament/physiology ; Periodontium/drug effects* ; Periodontium/physiology ; Recombinant Proteins
Keywords
dog ; periodontal regeneration ; rhGDF‐5 ; tissue engineering ; β‐TCP
Abstract
OBJECTIVE: Recombinant human growth/differentiation factor-5 (rhGDF-5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following the application of rhGDF-5 on a particulate beta-tricalcium phosphate (beta-TCP) carrier using an established defect model. MATERIALS AND METHODS: Bilateral 4 x 5 mm (width x depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in 15 Beagle dogs. Unilateral defects in five animals received rhGDF-5/beta-TCP (Scil Technology GmbH); five animals received beta-TCP solo; and five animals served as sham-surgery controls. Contralateral sites received treatments reported elsewhere. The animals were sacrificed following an 8-week healing interval for histological examination. RESULTS: Clinical healing was generally uneventful. Sites implanted with rhGDF-5/beta-TCP exhibited greater enhanced cementum and bone formation compared with beta-TCP and sham-surgery controls; cementum regeneration averaged (+/- SD) 3.83 +/- 0.73 versus 1.65 +/- 0.82 and 2.48 +/- 1.28 mm for the controls (p<0.05). Corresponding values for bone regeneration height averaged 3.26 +/- 0.30 versus 1.70 +/- 0.66 and 1.68 +/- 0.49 mm (p<0.05), and bone area 10.45 +/- 2.26 versus 6.31 +/- 2.41 and 3.00 +/- 1.97 mm(2) (p<0.05). Cementum regeneration included cellular/acellular cementum with or without a functionally oriented periodontal ligament. A non-specific connective tissue attachment was evident in the sham-surgery control. Controls exhibited mostly woven bone with primary osteons, whereas rhGDF-5/beta-TCP sites showed a noticeable extent of lamellar bone. Sites receiving rhGDF-5/beta-TCP or beta-TCP showed some residual beta-TCP granules apparently undergoing biodegradation without obvious differences between the sites. Sites receiving beta-TCP alone commonly showed residual beta-TCP granules sequestered in the connective tissue or fibrovascular marrow. CONCLUSION: rhGDF-5/beta-TCP has a greater potential to support the regeneration of the periodontal attachment. Long-term studies are necessary to confirm the uneventful maturation of the regenerated tissues.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-051X.2010.01544.x/abstract
DOI
10.1111/j.1600-051X.2010.01544.x
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Lee, Jung Seok(이정석)
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100983
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