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In vitro osteoblast differentiation is negatively regulated by Hoxc8

Authors
 Yan-Jun Zheng  ;  Hyun Joo Chung  ;  Hyehyun Min  ;  Myengmo Kang  ;  Seong Hwan Kim  ;  Jogeswar Gadi  ;  Myoung Hee Kim 
Citation
 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, Vol.160(3) : 891-900, 2010 
Journal Title
 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 
ISSN
 0273-2289 
Issue Date
2010
MeSH
Animals ; Bone Morphogenetic Proteins/pharmacology ; Cell Differentiation*/drug effects ; Cell Line ; Clone Cells ; Gene Expression Regulation ; Gene Knockdown Techniques ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism* ; Humans ; Mice ; Osteoblasts/cytology* ; Osteoblasts/drug effects ; Osteogenesis/drug effects ; RNA, Small Interfering/genetics ; Transfection
Keywords
Hoxc8 ; Osteoblast ; differentiation ; C2C12 ; MC3T3-E1 ; BMP signaling
Abstract
Hoxc8 has multiple roles in normal skeletal development. In this paper, a MC3T3-E1 subclone 4 osteogenic cell differentiation model was used to examine expression of Hoxc8 at multiple stages of osteogenesis. We found that Hoxc8 expression levels do not change in the early stage but increase in the middle stage and decrease in the late stage of osteogenesis. A knockdown of Hoxc8 by small-interfering RNA transfection in C2C12 cells indicated that Hoxc8 is a negative regulator of osteogenesis. Similarly, expression of Hoxc8 in C2C12 cells decreases alkaline phosphatase levels induced by bone morphogenetic protein-2 (BMP-2). The results of this study showed that Hoxc8 is involved in BMP-2-induced osteogenesis, and osteoblast differentiation in vitro is negatively regulated by Hoxc8, suggesting that Hoxc8 regulation is essential for osteoblast differentiation
Full Text
http://link.springer.com/article/10.1007%2Fs12010-009-8558-3
DOI
10.1007/s12010-009-8558-3
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kang, Myengmo(강명모)
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Min, Hye Hyun(민혜현) ORCID logo https://orcid.org/0000-0001-5450-5088
Chung, Huyun Joo(정현주)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100514
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