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Comparisons of three automated systems for genomic DNA extraction in a clinical diagnostic laboratory

 Jong-Han Lee  ;  Yongjung Park  ;  Jong Rak Choi  ;  Eun Kyung Lee  ;  Hyon-Suk Kim 
 YONSEI MEDICAL JOURNAL, Vol.51(1) : 104-110, 2010 
Journal Title
Issue Date
Automation/methods ; DNA/blood ; DNA/isolation & purification* ; Humans ; Polymerase Chain Reaction ; Reagent Kits,Diagnostic* ; Reproducibility of Results
Automatedsystem ; DNA ; nucleic acidextraction ; polymerase chain reaction
PURPOSE: The extraction of nucleic acid is initially a limiting step for successful molecular-based diagnostic workup. This study aims to compare the effectiveness of three automated DNA extraction systems for clinical laboratory use.

MATERIALS AND METHODS: Venous blood samples from 22 healthy volunteers were analyzed using QIAamp Blood Mini Kit (Qiagen), MagNA Pure LC Nucleic Acid Isolation Kit I (Roche), and Magtration-Magnazorb DNA common kit-200N (PSS). The concentration of extracted DNAs was measured by NanoDrop ND-1000 (PeqLab). Also, extracted DNAs were confirmed by applying in direct agarose gel electrophoresis and were amplified by polymerase chain reaction (PCR) for human beta-globin gene.

RESULTS: The corrected concentrations of extracted DNAs were 25.42 +/- 8.82 ng/microL (13.49-52.85 ng/microL) by QIAamp Blood Mini Kit (Qiagen), and 22.65 +/- 14.49 ng/microL (19.18-93.39 ng/microL) by MagNA Pure LC Nucleic Acid Isolation Kit I, and 22.35 +/- 6.47 ng/microL (12.57-35.08 ng/microL) by Magtration-Magnazorb DNA common kit-200N (PSS). No statistically significant difference was noticed among the three commercial kits (p > 0.05). Only the mean value of DNA purity through PSS was slightly lower than others. All the extracted DNAs were successfully identified in direct agarose gel electrophoresis. And all the product of beta-globin gene PCR showed a reproducible pattern of bands.

CONCLUSION: The effectiveness of the three automated extraction systems is of an equivalent level and good enough to produce reasonable results. Each laboratory could select the automated system according to its clinical and laboratory conditions
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정) ORCID logo https://orcid.org/0000-0001-5668-4120
Lee, Jong Han(이종한)
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
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