201 275

Cited 35 times in

Comparisons of three automated systems for genomic DNA extraction in a clinical diagnostic laboratory

DC FieldValueLanguage
dc.contributor.author최종락-
dc.contributor.author김현숙-
dc.contributor.author박용정-
dc.contributor.author이종한-
dc.date.accessioned2015-04-23T16:21:44Z-
dc.date.available2015-04-23T16:21:44Z-
dc.date.issued2010-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100444-
dc.description.abstractPURPOSE: The extraction of nucleic acid is initially a limiting step for successful molecular-based diagnostic workup. This study aims to compare the effectiveness of three automated DNA extraction systems for clinical laboratory use. MATERIALS AND METHODS: Venous blood samples from 22 healthy volunteers were analyzed using QIAamp Blood Mini Kit (Qiagen), MagNA Pure LC Nucleic Acid Isolation Kit I (Roche), and Magtration-Magnazorb DNA common kit-200N (PSS). The concentration of extracted DNAs was measured by NanoDrop ND-1000 (PeqLab). Also, extracted DNAs were confirmed by applying in direct agarose gel electrophoresis and were amplified by polymerase chain reaction (PCR) for human beta-globin gene. RESULTS: The corrected concentrations of extracted DNAs were 25.42 +/- 8.82 ng/microL (13.49-52.85 ng/microL) by QIAamp Blood Mini Kit (Qiagen), and 22.65 +/- 14.49 ng/microL (19.18-93.39 ng/microL) by MagNA Pure LC Nucleic Acid Isolation Kit I, and 22.35 +/- 6.47 ng/microL (12.57-35.08 ng/microL) by Magtration-Magnazorb DNA common kit-200N (PSS). No statistically significant difference was noticed among the three commercial kits (p > 0.05). Only the mean value of DNA purity through PSS was slightly lower than others. All the extracted DNAs were successfully identified in direct agarose gel electrophoresis. And all the product of beta-globin gene PCR showed a reproducible pattern of bands. CONCLUSION: The effectiveness of the three automated extraction systems is of an equivalent level and good enough to produce reasonable results. Each laboratory could select the automated system according to its clinical and laboratory conditions-
dc.description.statementOfResponsibilityopen-
dc.format.extent104~110-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAutomation/methods-
dc.subject.MESHDNA/blood-
dc.subject.MESHDNA/isolation & purification*-
dc.subject.MESHHumans-
dc.subject.MESHPolymerase Chain Reaction-
dc.subject.MESHReagent Kits, Diagnostic*-
dc.subject.MESHReproducibility of Results-
dc.titleComparisons of three automated systems for genomic DNA extraction in a clinical diagnostic laboratory-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorJong-Han Lee-
dc.contributor.googleauthorYongjung Park-
dc.contributor.googleauthorJong Rak Choi-
dc.contributor.googleauthorEun Kyung Lee-
dc.contributor.googleauthorHyon-Suk Kim-
dc.identifier.doi10.3349/ymj.2010.51.1.104-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04182-
dc.contributor.localIdA01582-
dc.contributor.localIdA03151-
dc.contributor.localIdA01117-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid20046522-
dc.subject.keywordAutomated system-
dc.subject.keyword DNA-
dc.subject.keywordnucleic acid extraction-
dc.subject.keywordpolymerase chain reaction-
dc.contributor.alternativeNameChoi, Jong Rak-
dc.contributor.alternativeNameKim, Hyon Suk-
dc.contributor.alternativeNamePark, Yong Jung-
dc.contributor.alternativeNameLee, Jong Han-
dc.contributor.affiliatedAuthorChoi, Jong Rak-
dc.contributor.affiliatedAuthorPark, Yong Jung-
dc.contributor.affiliatedAuthorLee, Jong Han-
dc.contributor.affiliatedAuthorKim, Hyon Suk-
dc.citation.volume51-
dc.citation.number1-
dc.citation.startPage104-
dc.citation.endPage110-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.51(1) : 104-110, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.