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Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells

Authors
 Young-Suk Kim  ;  Kyung-San Min  ;  Sang-Im Lee  ;  Su-Jung Shin  ;  Kyoung-Seob Shin  ;  Eun-Cheol Kim 
Citation
 JOURNAL OF ENDODONTICS, Vol.36(1) : 64-69, 2010 
Journal Title
JOURNAL OF ENDODONTICS
ISSN
 0099-2399 
Issue Date
2010
MeSH
AMP-Activated Protein Kinases/antagonists & inhibitors ; AMP-Activated Protein Kinases/metabolism ; Cell Line, Transformed ; Cytokines/physiology* ; DentalPulp/cytology ; DentalPulp/drug effects ; DentalPulp/immunology* ; DentalPulp/metabolism* ; GeneExpression ; Gene Knockdown Techniques ; Humans ; Inflammation Mediators/pharmacology* ; Interleukin-1alpha/pharmacology ; Interleukin-1alpha/physiology ; MAP Kinase Signaling System ; NF-kappa B/antagonists & inhibitors ; NF-kappa B/metabolism ; Protein Kinase C/antagonists & inhibitors ; Protein Kinase C/metabolism ; Recombinant Proteins/pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Necrosis Factor-alpha/pharmacology ; Tumor Necrosis Factor-alpha/physiology ; Up-Regulation ; beta-Defensins/genetics ; beta-Defensins/metabolism*
Keywords
Adenosine monophosphate-activated protein kinase ; human β-defensin 2 ; human dental pulp cells ; nuclear factor-κB ; protein kinase C
Abstract
INTRODUCTION: Although the expression of human beta-defensin-2 (hBD-2) in odontoblasts from human dental pulp (HDP) has been reported, the production of hBD-2 and its regulation remains poorly understood. The aim of this study was to investigate the effect of cytokines on the induction of hBD-2 and its signaling mechanisms in HDP cells.

METHODS: After stimulation with tumor necrosis factor alpha (TNF-alpha) and interleukin 1 alpha (IL-1 alpha), reverse-transcriptase polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay experiments were performed to evaluate the effects of these cytokines on the production of hBD-2.

RESULTS: TNF-alpha and IL-1 alpha synergistically increased hBD-2 messenger RNA levels, protein expression, and activity. The up-regulation of hBD-2 by cytokines was attenuated by pretreatment with inhibitors of PKC, JNK, p38, ERK MAPK, nuclear factor-kappaB, and adenosine monophosphate-activated protein kinase (AMPK).

CONCLUSION: These results suggest that TNF-alpha and IL-1 alpha up-regulate HBD-2 expression in HDP cells through the PKC, JNK MAPK, p38, ERK, NF-kappaB, and AMPK pathways. Thus, the induction of hBD-2 by proinflammatory cytokines might up-regulate the pulpal host immune defense system.
Full Text
http://www.sciencedirect.com/science/article/pii/S0099239909007821
DOI
10.1016/j.joen.2009.09.022
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100415
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