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Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells

DC Field Value Language
dc.contributor.author신수정-
dc.date.accessioned2015-04-23T16:20:46Z-
dc.date.available2015-04-23T16:20:46Z-
dc.date.issued2010-
dc.identifier.issn0099-2399-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100415-
dc.description.abstractINTRODUCTION: Although the expression of human beta-defensin-2 (hBD-2) in odontoblasts from human dental pulp (HDP) has been reported, the production of hBD-2 and its regulation remains poorly understood. The aim of this study was to investigate the effect of cytokines on the induction of hBD-2 and its signaling mechanisms in HDP cells. METHODS: After stimulation with tumor necrosis factor alpha (TNF-alpha) and interleukin 1 alpha (IL-1 alpha), reverse-transcriptase polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay experiments were performed to evaluate the effects of these cytokines on the production of hBD-2. RESULTS: TNF-alpha and IL-1 alpha synergistically increased hBD-2 messenger RNA levels, protein expression, and activity. The up-regulation of hBD-2 by cytokines was attenuated by pretreatment with inhibitors of PKC, JNK, p38, ERK MAPK, nuclear factor-kappaB, and adenosine monophosphate-activated protein kinase (AMPK). CONCLUSION: These results suggest that TNF-alpha and IL-1 alpha up-regulate HBD-2 expression in HDP cells through the PKC, JNK MAPK, p38, ERK, NF-kappaB, and AMPK pathways. Thus, the induction of hBD-2 by proinflammatory cytokines might up-regulate the pulpal host immune defense system.-
dc.description.statementOfResponsibilityopen-
dc.format.extent64~69-
dc.relation.isPartOfJOURNAL OF ENDODONTICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAMP-Activated Protein Kinases/antagonists & inhibitors-
dc.subject.MESHAMP-Activated Protein Kinases/metabolism-
dc.subject.MESHCell Line, Transformed-
dc.subject.MESHCytokines/physiology*-
dc.subject.MESHDentalPulp/cytology-
dc.subject.MESHDentalPulp/drug effects-
dc.subject.MESHDentalPulp/immunology*-
dc.subject.MESHDentalPulp/metabolism*-
dc.subject.MESHGeneExpression-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHHumans-
dc.subject.MESHInflammation Mediators/pharmacology*-
dc.subject.MESHInterleukin-1alpha/pharmacology-
dc.subject.MESHInterleukin-1alpha/physiology-
dc.subject.MESHMAP Kinase Signaling System-
dc.subject.MESHNF-kappa B/antagonists & inhibitors-
dc.subject.MESHNF-kappa B/metabolism-
dc.subject.MESHProtein Kinase C/antagonists & inhibitors-
dc.subject.MESHProtein Kinase C/metabolism-
dc.subject.MESHRecombinant Proteins/pharmacology-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHTumor Necrosis Factor-alpha/pharmacology-
dc.subject.MESHTumor Necrosis Factor-alpha/physiology-
dc.subject.MESHUp-Regulation-
dc.subject.MESHbeta-Defensins/genetics-
dc.subject.MESHbeta-Defensins/metabolism*-
dc.titleEffect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Conservative Dentistry (보존과학)-
dc.contributor.googleauthorYoung-Suk Kim-
dc.contributor.googleauthorKyung-San Min-
dc.contributor.googleauthorSang-Im Lee-
dc.contributor.googleauthorSu-Jung Shin-
dc.contributor.googleauthorKyoung-Seob Shin-
dc.contributor.googleauthorEun-Cheol Kim-
dc.identifier.doi10.1016/j.joen.2009.09.022-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02117-
dc.relation.journalcodeJ01393-
dc.identifier.eissn1878-3554-
dc.identifier.pmid20003937-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0099239909007821-
dc.subject.keywordAdenosine monophosphate-activated protein kinase-
dc.subject.keywordhuman β-defensin 2-
dc.subject.keywordhuman dental pulp cells-
dc.subject.keywordnuclear factor-κB-
dc.subject.keywordprotein kinase C-
dc.contributor.alternativeNameShin, Su Jung-
dc.contributor.affiliatedAuthorShin, Su Jung-
dc.citation.volume36-
dc.citation.number1-
dc.citation.startPage64-
dc.citation.endPage69-
dc.identifier.bibliographicCitationJOURNAL OF ENDODONTICS, Vol.36(1) : 64-69, 2010-
dc.identifier.rimsid36472-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers

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