Cited 31 times in
Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells
DC Field | Value | Language |
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dc.contributor.author | 신수정 | - |
dc.date.accessioned | 2015-04-23T16:20:46Z | - |
dc.date.available | 2015-04-23T16:20:46Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0099-2399 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100415 | - |
dc.description.abstract | INTRODUCTION: Although the expression of human beta-defensin-2 (hBD-2) in odontoblasts from human dental pulp (HDP) has been reported, the production of hBD-2 and its regulation remains poorly understood. The aim of this study was to investigate the effect of cytokines on the induction of hBD-2 and its signaling mechanisms in HDP cells. METHODS: After stimulation with tumor necrosis factor alpha (TNF-alpha) and interleukin 1 alpha (IL-1 alpha), reverse-transcriptase polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay experiments were performed to evaluate the effects of these cytokines on the production of hBD-2. RESULTS: TNF-alpha and IL-1 alpha synergistically increased hBD-2 messenger RNA levels, protein expression, and activity. The up-regulation of hBD-2 by cytokines was attenuated by pretreatment with inhibitors of PKC, JNK, p38, ERK MAPK, nuclear factor-kappaB, and adenosine monophosphate-activated protein kinase (AMPK). CONCLUSION: These results suggest that TNF-alpha and IL-1 alpha up-regulate HBD-2 expression in HDP cells through the PKC, JNK MAPK, p38, ERK, NF-kappaB, and AMPK pathways. Thus, the induction of hBD-2 by proinflammatory cytokines might up-regulate the pulpal host immune defense system. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 64~69 | - |
dc.relation.isPartOf | JOURNAL OF ENDODONTICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | AMP-Activated Protein Kinases/antagonists & inhibitors | - |
dc.subject.MESH | AMP-Activated Protein Kinases/metabolism | - |
dc.subject.MESH | Cell Line, Transformed | - |
dc.subject.MESH | Cytokines/physiology* | - |
dc.subject.MESH | DentalPulp/cytology | - |
dc.subject.MESH | DentalPulp/drug effects | - |
dc.subject.MESH | DentalPulp/immunology* | - |
dc.subject.MESH | DentalPulp/metabolism* | - |
dc.subject.MESH | GeneExpression | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation Mediators/pharmacology* | - |
dc.subject.MESH | Interleukin-1alpha/pharmacology | - |
dc.subject.MESH | Interleukin-1alpha/physiology | - |
dc.subject.MESH | MAP Kinase Signaling System | - |
dc.subject.MESH | NF-kappa B/antagonists & inhibitors | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Protein Kinase C/antagonists & inhibitors | - |
dc.subject.MESH | Protein Kinase C/metabolism | - |
dc.subject.MESH | Recombinant Proteins/pharmacology | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/pharmacology | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/physiology | - |
dc.subject.MESH | Up-Regulation | - |
dc.subject.MESH | beta-Defensins/genetics | - |
dc.subject.MESH | beta-Defensins/metabolism* | - |
dc.title | Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Conservative Dentistry (보존과학) | - |
dc.contributor.googleauthor | Young-Suk Kim | - |
dc.contributor.googleauthor | Kyung-San Min | - |
dc.contributor.googleauthor | Sang-Im Lee | - |
dc.contributor.googleauthor | Su-Jung Shin | - |
dc.contributor.googleauthor | Kyoung-Seob Shin | - |
dc.contributor.googleauthor | Eun-Cheol Kim | - |
dc.identifier.doi | 10.1016/j.joen.2009.09.022 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02117 | - |
dc.relation.journalcode | J01393 | - |
dc.identifier.eissn | 1878-3554 | - |
dc.identifier.pmid | 20003937 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0099239909007821 | - |
dc.subject.keyword | Adenosine monophosphate-activated protein kinase | - |
dc.subject.keyword | human β-defensin 2 | - |
dc.subject.keyword | human dental pulp cells | - |
dc.subject.keyword | nuclear factor-κB | - |
dc.subject.keyword | protein kinase C | - |
dc.contributor.alternativeName | Shin, Su Jung | - |
dc.contributor.affiliatedAuthor | Shin, Su Jung | - |
dc.citation.volume | 36 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 64 | - |
dc.citation.endPage | 69 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ENDODONTICS, Vol.36(1) : 64-69, 2010 | - |
dc.identifier.rimsid | 36472 | - |
dc.type.rims | ART | - |
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