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인간 섬유아세포에 대한 마이토마이신 C와 피르페니돈의독성과 항섬유화효과

Other Titles
 Cytotoxicities and Anti-Fibrotic Effects of Pirfenidone and Mitomycin C on Human Fibroblasts 
Authors
 박경수  ;  홍사민  ;  이주카요코  ;  김찬윤  ;  성공제 
Citation
 Journal of the Korean Ophthalmological Society (대한안과학회지), Vol.55(7) : 1077-1083, 2014 
Journal Title
 Journal of the Korean Ophthalmological Society (대한안과학회지) 
ISSN
 0378-6471 
Issue Date
2014
Keywords
Anti-fibrotic effect ; Cytotoxicity ; Mitomycin C ; Pirfenidone
Abstract
Purpose: The cytotoxicities and anti-fibrotic effects of mitomycin C and pirfenidone on human dermal fibroblast were evaluated. Methods: Initially, 24-hour cell cultures were exposed to transforming growth factor (TGF)-β1, different concentrations of mitomycin C, and pirfenidone solutions in order to evaluate cytotoxicity. Expressions of fibronectin, collagen type 1, α smooth muscle, and β-actin were evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blot in mitomycin C solutions at concentrations of 4 μg/mL and 20 μg/mL, and in pirfenidone solutions at 250 μg/mL and 500 μg/mL. Results: In comparison to cell cultures exposed to TGF-β1 solutions, cytotoxicities were increased in solutions of mitomycin C at 4 μg/mL, 20 μg/mL, 40 μg/mL and pirfenidone at 500 μg/mL, 750 μg/mL, 1,000 μg/mL (p < 0.05, Mann Whitney U-test). The results of real-time RT-PCR show that expressions of fibronectin, collagen type 1, and α smooth muscle were significantly more decreased in all concentrations of mitomycin C and pirfenidone compared to those in TGF-β1 solution. In western blot analysis, expressions of fibronectin and α smooth muscle were decreased in all concentrations of mitomycin C and pirfenidone compared to TGF-β1 solution. Conclusions: Both drugs have cytotoxicities and anti-fibrotic effects, but pirfenidone was found to have less cytotoxicity and mitomycin C was found to have more anti-fibrotic effects when compared to each other.
Files in This Item:
T201404095.pdf Download
DOI
10.3341/jkos.2014.55.7.1077
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Chan Yun(김찬윤) ORCID logo https://orcid.org/0000-0002-8373-9999
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
Iizuka, Yoko(이주카요코)
Hong, Sa Min(홍사민)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100338
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