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인간 섬유아세포에 대한 마이토마이신 C와 피르페니돈의독성과 항섬유화효과
DC Field | Value | Language |
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dc.contributor.author | 이주카요코 | - |
dc.contributor.author | 홍사민 | - |
dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 성공제 | - |
dc.date.accessioned | 2015-01-06T17:37:52Z | - |
dc.date.available | 2015-01-06T17:37:52Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0378-6471 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100338 | - |
dc.description.abstract | Purpose: The cytotoxicities and anti-fibrotic effects of mitomycin C and pirfenidone on human dermal fibroblast were evaluated. Methods: Initially, 24-hour cell cultures were exposed to transforming growth factor (TGF)-β1, different concentrations of mitomycin C, and pirfenidone solutions in order to evaluate cytotoxicity. Expressions of fibronectin, collagen type 1, α smooth muscle, and β-actin were evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blot in mitomycin C solutions at concentrations of 4 μg/mL and 20 μg/mL, and in pirfenidone solutions at 250 μg/mL and 500 μg/mL. Results: In comparison to cell cultures exposed to TGF-β1 solutions, cytotoxicities were increased in solutions of mitomycin C at 4 μg/mL, 20 μg/mL, 40 μg/mL and pirfenidone at 500 μg/mL, 750 μg/mL, 1,000 μg/mL (p < 0.05, Mann Whitney U-test). The results of real-time RT-PCR show that expressions of fibronectin, collagen type 1, and α smooth muscle were significantly more decreased in all concentrations of mitomycin C and pirfenidone compared to those in TGF-β1 solution. In western blot analysis, expressions of fibronectin and α smooth muscle were decreased in all concentrations of mitomycin C and pirfenidone compared to TGF-β1 solution. Conclusions: Both drugs have cytotoxicities and anti-fibrotic effects, but pirfenidone was found to have less cytotoxicity and mitomycin C was found to have more anti-fibrotic effects when compared to each other. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1077~1083 | - |
dc.relation.isPartOf | JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 인간 섬유아세포에 대한 마이토마이신 C와 피르페니돈의독성과 항섬유화효과 | - |
dc.title.alternative | Cytotoxicities and Anti-Fibrotic Effects of Pirfenidone and Mitomycin C on Human Fibroblasts | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | 박경수 | - |
dc.contributor.googleauthor | 홍사민 | - |
dc.contributor.googleauthor | 이주카요코 | - |
dc.contributor.googleauthor | 김찬윤 | - |
dc.contributor.googleauthor | 성공제 | - |
dc.identifier.doi | 10.3341/jkos.2014.55.7.1077 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03162 | - |
dc.contributor.localId | A04395 | - |
dc.contributor.localId | A01035 | - |
dc.contributor.localId | A01946 | - |
dc.relation.journalcode | J01838 | - |
dc.identifier.eissn | 2092-9374 | - |
dc.subject.keyword | Anti-fibrotic effect | - |
dc.subject.keyword | Cytotoxicity | - |
dc.subject.keyword | Mitomycin C | - |
dc.subject.keyword | Pirfenidone | - |
dc.contributor.alternativeName | Iizuka, Yoko | - |
dc.contributor.alternativeName | Hong, Sa Min | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Iizuka, Yoko | - |
dc.contributor.affiliatedAuthor | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Kim, Chan Yun | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.citation.volume | 55 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1077 | - |
dc.citation.endPage | 1083 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY, Vol.55(7) : 1077-1083, 2014 | - |
dc.identifier.rimsid | 57600 | - |
dc.type.rims | ART | - |
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