Autophagy Activity in Pulmonary Metastatic Tumor Tissues from Colorectal Cancer: A Pilot Study

374 760

Cited 8 times in

Authors: Young Sam Kim ; Ju Hye Shin ; Mi Kyung Bae ; Chang Young Lee ; Dae Joon Kim ; Kyung Young Chung ; Jin Gu Lee

MeSH: Adaptor Proteins, Signal Transducing ; Aged ; Autophagy* ; Biomarkers, Tumor/metabolism ; Blotting, Western ; Colorectal Neoplasms/metabolism* ; Colorectal Neoplasms/pathology* ; Female ; Humans ; Lung Neoplasms/metabolism* ; Lung Neoplasms/pathology* ; Male ; Microfilament Proteins/metabolism* ; Microscopy, Electron, Transmission ; Microtubule-Associated Proteins ; Middle Aged ; Neoplasm Recurrence, Local ; Pilot Projects ; Proteins ; Retrospective Studies ; Sequestosome-1 Protein ; Treatment Failure

Abstract: PURPOSE:
Autophagy has been reported to be involved in treatment failure in tumor. We aimed to evaluate autophagy activity in tumor tissue and compare them between the recurrence and non-recurrence groups.
MATERIALS AND METHODS:
We analyzed expressions of autophagy-related proteins in tumor tissues which were obtained from pulmonary metastases of colorectal cancer patients by Western blot. We also analyzed autophagosomes by transmission electron microscopy.
RESULTS:
Tumor tissues from recurrence group showed increased levels of LC3B-II, decreased levels of p62/SQSTM1, and also a marked accumulation of autophagosomes compared with tissues from non-recurrence group.
CONCLUSION:
The present study suggests that autophagy may be associated with treatment failure of metastatic colorectal cancer.