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Characterization of a novel antigen of Mycobacterium tuberculosis K strain and its use in immunodiagnosis of tuberculosis

 Paul J. Park  ;  Ah Reum Kim  ;  Yangkyo P. Salch  ;  Taeksun Song  ;  Sung Jae Shin  ;  Seung Jung Han  ;  Sang-Nae Cho 
 JOURNAL OF MICROBIOLOGY, Vol.52(10) : 871-878, 2014 
Journal Title
Issue Date
Animals ; Antibodies, Bacterial/blood* ; Antigens, Bacterial*/genetics ; Antigens, Bacterial*/immunology ; Cloning, Molecular ; Escherichia coli/genetics ; Humans ; Immunologic Tests/methods* ; Interferon-gamma/secretion ; Interleukin-17/secretion ; Leukocytes, Mononuclear/immunology ; Mice, Inbred BALB C ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/immunology* ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Spleen/immunology ; Tuberculosis/diagnosis*
Mycobacterium tuberculosis ; K strain ; ESAT-6-like protein ; InsB ; immunodiagnosis
Mycobacterium tuberculosis-specific antigens would be of great value in developing immunodiagnostic tests for tuberculosis (TB), but regional differences in molecular types of the organism may result in antigenic variation, which in turn affects the outcome of the tests. For example, the Beijing strains of M. tuberculosis are prevalent in East Asia, and in particular, the K strain and related strains of the Beijing family, are most frequently isolated during school outbreaks of TB in South Korea. From comparison of genome sequences between M. tuberculosis K strain and the H37Rv strain, a non-Beijing type, we identified a K strain-specific gene, InsB, which has substantial homology with the ESAT-6-like proteins. This study was, therefore, initiated to characterize the InsB protein for its immunogenicity in mice and to confirm its expression in TB patients by detecting antibodies to the protein. The InsB gene was cloned from M. tuberculosis K strain and expressed in Escherichia coli. The recombinant InsB protein was used for immunization of mice. All mice showed strong antibody responses to the InsB protein, and splenocytes stimulated with InsB showed strong IFN-γ and IL-17 responses and a weak IL-2 response, all of which have been implicated in disease expression and used for the immunodiagnosis of TB. Serum samples from TB patients also showed significant antibody responses to the InsB protein as compared to healthy control samples. These results indicate that the InsB protein is an M. tuberculosis K-strain-specific antigen that could further improve the current immunodiagnostic methods, especially for the South Korean population.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Genome Research Center for Respiratory Pathogens (호흡기계감염세균유전체연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Ah Reum(김아름) ORCID logo https://orcid.org/0000-0001-5938-4270
Song, Taek Sun(송택선)
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
Cho, Sang Nae(조상래)
Han, Seung Jung(한승정)
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