1 200

Cited 6 times in

Proteomic analysis of CD44(+) and CD44(−) gastric cancer cells

Authors
 Dayeon Yu  ;  Hyun-Soo Shin  ;  Go Choi  ;  Yong Chan Lee 
Citation
 MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol.396(1-2) : 213-220, 2014 
Journal Title
 MOLECULAR AND CELLULAR BIOCHEMISTRY 
ISSN
 0300-8177 
Issue Date
2014
MeSH
Biomarkers, Tumor/metabolism ; Cell Separation ; Cytoskeletal Proteins/metabolism ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Profiling ; HSC70 Heat-Shock Proteins/metabolism ; Humans ; Hyaluronan Receptors/metabolism* ; Neoplastic Stem Cells/metabolism ; Phosphopyruvate Hydratase/metabolism ; Proteins/analysis* ; Proteins/genetics ; Proteins/metabolism ; Proteomics/methods ; Reproducibility of Results ; Stomach Neoplasms/metabolism* ; Stomach Neoplasms/pathology ; Up-Regulation
Keywords
Cancer stem cells ; CD44 ; Gastric cancer ; 2-DE
Abstract
CD44 is a cell surface protein and it is widely used as a cancer stem cell marker in various cancer types including gastric cancer. We conducted proteomic analysis in CD44(+) and CD44(−) gastric cancer cells to understand characteristics of CD44(+) and CD44(−) cells. In the present study, we sorted cells from the gastric cancer cell line MKN45 according to CD44 expression to separate out CD44(+) and CD44(−) cells. And we conducted RT-PCR to identify mRNA expression of cancer stem cell markers in CD44(+) and CD44(−) cells. Cancer stem cell markers showed upregulated expression in CD44(+) cells. Next, we performed two-dimensional electrophoresis analysis to determine the differential expression pattern of proteins in each group; control, CD44(+), and CD44(−) MKN45 cells. We found a total of 113 spots that varied in expression between CD44(+) and CD44(−) cells, and subjected 20 of those protein spots to MALDI-MS. We selected the three proteins (HSPA8; heat shock cognate 71 kDa protein isoform 1, ezrin, α-enolase) upregulated in CD44(+) cells than CD44(−) cells and one protein (prohibitin) showed increased expression in CD44(−) cells. We validated the protein expression levels of four selected proteins by Western blot. We suggest that our study could be a helpful background to study CD44(+) cancer stem-like cells and differences between CD44(+) and CD44(−) cells in gastric cancer.
Full Text
http://link.springer.com/article/10.1007%2Fs11010-014-2156-6
DOI
10.1007/s11010-014-2156-6
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Yu, Da Yeon(유다연)
Lee, Yong Chan(이용찬) ORCID logo https://orcid.org/0000-0001-8800-6906
Choi, Go(최고)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99750
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse