Cited 8 times in
Proteomic analysis of CD44(+) and CD44(−) gastric cancer cells
DC Field | Value | Language |
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dc.contributor.author | 유다연 | - |
dc.contributor.author | 이용찬 | - |
dc.contributor.author | 최고 | - |
dc.date.accessioned | 2015-01-06T17:18:59Z | - |
dc.date.available | 2015-01-06T17:18:59Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0300-8177 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99750 | - |
dc.description.abstract | CD44 is a cell surface protein and it is widely used as a cancer stem cell marker in various cancer types including gastric cancer. We conducted proteomic analysis in CD44(+) and CD44(−) gastric cancer cells to understand characteristics of CD44(+) and CD44(−) cells. In the present study, we sorted cells from the gastric cancer cell line MKN45 according to CD44 expression to separate out CD44(+) and CD44(−) cells. And we conducted RT-PCR to identify mRNA expression of cancer stem cell markers in CD44(+) and CD44(−) cells. Cancer stem cell markers showed upregulated expression in CD44(+) cells. Next, we performed two-dimensional electrophoresis analysis to determine the differential expression pattern of proteins in each group; control, CD44(+), and CD44(−) MKN45 cells. We found a total of 113 spots that varied in expression between CD44(+) and CD44(−) cells, and subjected 20 of those protein spots to MALDI-MS. We selected the three proteins (HSPA8; heat shock cognate 71 kDa protein isoform 1, ezrin, α-enolase) upregulated in CD44(+) cells than CD44(−) cells and one protein (prohibitin) showed increased expression in CD44(−) cells. We validated the protein expression levels of four selected proteins by Western blot. We suggest that our study could be a helpful background to study CD44(+) cancer stem-like cells and differences between CD44(+) and CD44(−) cells in gastric cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 213~220 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Biomarkers, Tumor/metabolism | - |
dc.subject.MESH | Cell Separation | - |
dc.subject.MESH | Cytoskeletal Proteins/metabolism | - |
dc.subject.MESH | Electrophoresis, Gel, Two-Dimensional | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | HSC70 Heat-Shock Proteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hyaluronan Receptors/metabolism* | - |
dc.subject.MESH | Neoplastic Stem Cells/metabolism | - |
dc.subject.MESH | Phosphopyruvate Hydratase/metabolism | - |
dc.subject.MESH | Proteins/analysis* | - |
dc.subject.MESH | Proteins/genetics | - |
dc.subject.MESH | Proteins/metabolism | - |
dc.subject.MESH | Proteomics/methods | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Stomach Neoplasms/metabolism* | - |
dc.subject.MESH | Stomach Neoplasms/pathology | - |
dc.subject.MESH | Up-Regulation | - |
dc.title | Proteomic analysis of CD44(+) and CD44(−) gastric cancer cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Dayeon Yu | - |
dc.contributor.googleauthor | Hyun-Soo Shin | - |
dc.contributor.googleauthor | Go Choi | - |
dc.contributor.googleauthor | Yong Chan Lee | - |
dc.identifier.doi | 10.1007/s11010-014-2156-6 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02458 | - |
dc.contributor.localId | A02988 | - |
dc.contributor.localId | A04038 | - |
dc.relation.journalcode | J02242 | - |
dc.identifier.eissn | 1573-4919 | - |
dc.identifier.pmid | 25081334 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs11010-014-2156-6 | - |
dc.subject.keyword | Cancer stem cells | - |
dc.subject.keyword | CD44 | - |
dc.subject.keyword | Gastric cancer | - |
dc.subject.keyword | 2-DE | - |
dc.contributor.alternativeName | Yu, Da Yeon | - |
dc.contributor.alternativeName | Lee, Yong Chan | - |
dc.contributor.alternativeName | Choi, Go | - |
dc.contributor.affiliatedAuthor | Yu, Da Yeon | - |
dc.contributor.affiliatedAuthor | Lee, Yong Chan | - |
dc.contributor.affiliatedAuthor | Choi, Go | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 396 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 213 | - |
dc.citation.endPage | 220 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol.396(1-2) : 213-220, 2014 | - |
dc.identifier.rimsid | 57094 | - |
dc.type.rims | ART | - |
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