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Long non-coding RNA HOTAIR promotes carcinogenesis and invasion of gastric adenocarcinoma

 Na Keum Lee  ;  Jung Hwa Lee  ;  Chan Hyuk Park  ;  Dayeon Yu  ;  Yong Chan Lee  ;  Jae-Ho Cheong  ;  Sung Hoon Noh  ;  Sang Kil Lee 
 Biochemical and Biophysical Research Communications, Vol.45(1) : 171-178, 2014 
Journal Title
 Biochemical and Biophysical Research Communications 
Issue Date
Adenocarcinoma/etiology ; Adenocarcinoma/genetics* ; Adenocarcinoma/pathology ; Adult ; Aged ; Apoptosis ; Carcinogenesis/genetics ; Cell Cycle ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation ; Epithelial-Mesenchymal Transition/genetics ; Female ; Gene Expression ; Gene Knockdown Techniques ; Humans ; Lymphatic Metastasis/genetics ; Male ; Middle Aged ; Neoplasm Invasiveness/genetics ; Neoplasm Invasiveness/pathology ; RNA, Long Noncoding/antagonists & inhibitors ; RNA, Long Noncoding/genetics* ; RNA, Neoplasm/antagonists & inhibitors ; RNA, Neoplasm/genetics* ; RNA, Small Interfering/genetics ; Stomach Neoplasms/etiology ; Stomach Neoplasms/genetics* ; Stomach Neoplasms/pathology
Apoptosis ; Gastric cancer ; HOTAIR ; Invasion ; Metastasis
Gastric cancer is one of the major causes of cancer death worldwide; however, the mechanism of carcinogenesis is complex and poorly understood. Long non-coding RNA HOTAIR (HOX transcript antisense RNA) recently emerged as a promoter of metastasis in various cancers including gastric cancer. Here we investigated the impact of HOTAIR on apoptosis, cell proliferation and cell cycle to dissect the carcinogenesis of gastric cancer. We examined the mechanism of invasion and metastasis and analyzed the clinical significance of HOTAIR. Downregulation of HOTAIR was confirmed by two different siRNAs. The expression of HOTAIR was significantly elevated in various gastric cancer cell lines and tissues compared to normal control. si-HOTAIR significantly reduced viability in MKN 28, MKN 74, and KATO III cells but not in AGS cells. si-HOTAIR induced apoptosis in KATO III cells. Lymphovascular invasion and lymph node metastasis were more common in the high level of HOTAIR group. si-HOTAIR significantly decreased invasiveness and migration. si-HOTAIR led to differential expression of epithelial to mesenchymal transition markers. We found that HOTAIR was involved in inhibition of apoptosis and promoted invasiveness, supporting a role for HOTAIR in carcinogenesis and progression of gastric cancer.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Noh, Sung Hoon(노성훈) ORCID logo https://orcid.org/0000-0003-4386-6886
Park, Chan Hyuk(박찬혁)
Yu, Da Yeon(유다연)
Lee, Na Keum(이나금)
Lee, Sang Kil(이상길) ORCID logo https://orcid.org/0000-0002-0721-0364
Lee, Yong Chan(이용찬) ORCID logo https://orcid.org/0000-0001-8800-6906
Lee, Jung Hwa(이정화)
Cheong, Jae Ho(정재호) ORCID logo https://orcid.org/0000-0002-1703-1781
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