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The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury

Authors
 Juhyun Song  ;  So Mang Kang  ;  Won Taek Lee  ;  Kyung Ah Park  ;  Kyoung Min Lee  ;  Jong Eun Lee 
Citation
 OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, Vol.2014 : 639531, 2014 
Journal Title
 OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 
ISSN
 1942-0900 
Issue Date
2014
MeSH
Animals ; Antioxidants/pharmacology ; Apoptosis/drug effects* ; Brain/cytology ; Cell Line ; Endothelial Cells/cytology ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism* ; Gene Expression/drug effects ; Glucose/pharmacology* ; Hypoxia ; JNK Mitogen-Activated Protein Kinases/metabolism ; Melatonin/pharmacology* ; Mice ; Proto-Oncogene Proteins c-akt/metabolism ; Reactive Oxygen Species/metabolism ; Reperfusion Injury/metabolism ; Reperfusion Injury/pathology ; Tight Junction Proteins/metabolism ; Vascular Endothelial Growth Factor A/metabolism ; bcl-2-Associated X Protein/metabolism
Abstract
Melatonin has a cellular protective effect in cerebrovascular and neurodegenerative diseases. Protection of brain endothelial cells against hypoxia and oxidative stress is important for treatment of central nervous system (CNS) diseases, since brain endothelial cells constitute the blood brain barrier (BBB). In the present study, we investigated the protective effect of melatonin against oxygen-glucose deprivation, followed by reperfusion- (OGD/R-) induced injury, in bEnd.3 cells. The effect of melatonin was examined by western blot analysis, cell viability assays, measurement of intracellular reactive oxygen species (ROS), and immunocytochemistry (ICC). Our results showed that treatment with melatonin prevents cell death and degradation of tight junction protein in the setting of OGD/R-induced injury. In response to OGD/R injury of bEnd.3 cells, melatonin activates Akt, which promotes cell survival, and attenuates phosphorylation of JNK, which triggers apoptosis. Thus, melatonin protects bEnd.3 cells against OGD/R-induced injury.
Files in This Item:
T201402505.pdf Download
DOI
10.1155/2014/639531
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Ah(박경아)
Song, Ju Hyun(송주현)
Lee, Won Taek(이원택) ORCID logo https://orcid.org/0000-0001-7348-9562
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99374
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