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Short Communication: Prospective Comparison of Qualitative Versus Quantitative Polymerase Chain Reaction for Monitoring Virologic Treatment Failure in HIV-Infected Patients

Authors
 Jeong Su Jin  ;  Kim Min Hyung  ;  Song Je Eun  ;  Ahn Jin Young  ;  Kim Sun Bean  ;  Ann Hea Won  ;  Kim Jae Kyung  ;  Choi Heun  ;  Ku Nam Su  ;  Han Sang Hoon  ;  Kim June Myung  ;  Smith Davey M.  ;  Kim Hyon-Suk  ;  Choi Jun Yong 
Citation
 AIDS RESEARCH AND HUMAN RETROVIRUSES, Vol.30(8) : 827-829, 2014 
Journal Title
 AIDS RESEARCH AND HUMAN RETROVIRUSES 
ISSN
 0889-2229 
Issue Date
2014
MeSH
Adult ; Aged ; Aged, 80 and over ; Anti-HIV Agents/therapeutic use* ; Drug Monitoring/methods* ; Female ; HIV Infections/drug therapy* ; HIV Infections/virology* ; HIV-1/genetics ; HIV-1/isolation & purification* ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction/methods* ; Prospective Studies ; Republic of Korea ; Sensitivity and Specificity ; Treatment Failure ; Viral Load/methods* ; Young Adult
Abstract
Less costly but still accurate methods for monitoring HIV treatment response are needed. We prospectively evaluated if a qualitative polymerase chain reaction (PCR) amplification assay for virologic monitoring could maintain accuracy while reducing costs in Seoul, South Korea. We conducted the first prospective study comparing a qualitative PCR amplification of HIV-1 reverse transcriptase (RT) versus a commercial real time PCR assay (i.e., viral load) for virologic monitoring of 150 patients receiving antiretroviral therapy (ART) between November 2011 and August 2012 at an urban hospital in Seoul, South Korea. A total of 215 blood plasma samples from 150 patients receiving ART for more than 6 months were evaluated. Using the individual viral load assay, 12 of 215 (5.6%) plasma samples had more than 500 HIV RNA copies/ml. The qualitative PCR amplification assay detected individual samples with ≥500 HIV RNA copies/ml with 100% sensitivity. The specificities of the qualitative PCR amplification of the HIV-1 RT assay were 94.1%, 93.6%, and 93.2% compared to the real time PCR at 500, 1,000, and 5,000 threshold of HIV RNA copies/ml, respectively, and $24,940 USD would have been saved for 150 patients during 10 months. The qualitative PCR amplification of the HIV-1 RT assay might be a useful approach to effectively monitor patients receiving ART and save resources.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/aid.2013.0227
DOI
10.1089/aid.2013.0227
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Ku, Nam Su(구남수) ORCID logo https://orcid.org/0000-0002-9717-4327
Kim, Min Hyung(김민형)
Kim, Sun Bean(김선빈)
Kim, Jae Kyoung(김재경)
Kim, June Myung(김준명)
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Song, Je Eun(송제은)
Ahn, Jin Young(안진영) ORCID logo https://orcid.org/0000-0002-3740-2826
Ann, Hea Won(안혜원)
Jeong, Su Jin(정수진) ORCID logo https://orcid.org/0000-0003-4025-4542
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
Choi, Heun(최흔) ORCID logo https://orcid.org/0000-0002-9622-9381
Han, Sang Hoon(한상훈) ORCID logo https://orcid.org/0000-0002-4278-5198
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99371
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