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미세표면분석 기법을 이용한 미용 콘택트렌즈와 일반 콘택트렌즈의표면거칠기와 세균부착성 비교

Other Titles
 Comparison of Surface Roughness and Bacterial Adhesion between Cosmetic Contact Lenses and Conventional Contact Lenses 
Authors
 지용우  ;  홍순호  ;  정동룡  ;  김응권  ;  이형근 
Citation
 JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY, Vol.55(5) : 646-655, 2014 
Journal Title
 JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY 
ISSN
 0378-6471 
Issue Date
2014
MeSH
Bacterial Adhesion*/drug effects ; Bacterial Adhesion*/physiology ; Beauty Culture ; Biofilms/drug effects ; Biofilms/growth & development ; Colony Count, Microbial ; Contact Lenses, Hydrophilic/microbiology* ; Disinfectants/pharmacology ; Humans ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Ophthalmic Solutions/pharmacology ; Pseudomonas aeruginosa/physiology ; Staphylococcus aureus/physiology ; Surface Properties*
Keywords
Atomic force microscope ; Bacterial adhesion ; Cosmetic contact lens ; Pseudomonas aeruginosa ; Surface roughness
Abstract
Purpose: To determine the surface roughness of cosmetic and conventional contact lenses (CLs) and their susceptibility to bacterialadhesion. Methods: Concave surface roughness of cosmetic and conventional hydrogel (Etafilcon A) CLs was measured by atomic forcemicroscopy (AFM) and scanning electron microscopy (SEM). In particular, the surface of the color tinted area of cosmetic CLswas measured. CLs were immersed into a bacterial solution of Pseudomonas aeruginosa for 1, 12, or 24 hours and culture of P. aeruginosa that had adhered to the CLs was performed. Results: Concave surface roughness of cosmetic CLs significantly increased compared with conventional CLs by AFM (p < 0.05). Bacterial colony formation of P. aeruginosa adhering to cosmetic CLs within one hour significantly increased compared with conventionalCLs (p = 0.047). Adhesions of P. aeruginosa to CLs within one hour was found to correlate significantly with the surface roughness of CL(r > 0.9, p < 0.05). By SEM, P. aeruginosa had adhered to the color-tinted area more than to the non-color-tinted area of cosmetic CLs. Conclusions: Surface of cosmetic CLs was significantly rougher and initial adhesion of bacteria was higher to cosmetic CLs thanto conventional CLs. In particular, an increased number of bacteria was found to be adhered to the color-tinted area of cosmeticCLs. Initial bacterial adhesion is important because it is the first stage of bacterial attachment process to any surface. After then,the adherent bacteria can progress to form a biofilm. Increased surface roughness of CLs contributes to opportunities for the CLto come into contact with bacteria, and thus, initial bacterial adhesion increases. In this study, it is clear that cosmetic CLs aremore vulnerable to bacterial adhesion. To avoid serious complications, such as bacterial keratitis, the manufacturing process forsmoothing and treating the surface in order to inhibit bacterial adhesion should be developed in the future.
Files in This Item:
T201402425.pdf Download
DOI
10.3341/jkos.2014.55.5.646
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99329
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