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A Preclinical Rodent Model of Acute Radiation-Induced Lung Injury after Ablative Focal Irradiation Reflecting Clinical Stereotactic Body Radiotherapy

 Zhen-Yu Hong  ;  Hae-June Lee  ;  Won Hoon Choi  ;  Yoon-Jin Lee  ;  Sung Ho Eun  ;  Jung Il Lee  ;  Kwangwoo Park  ;  Ji Min Lee  ;  Jaeho Cho 
 RADIATION RESEARCH, Vol.182(1) : 83-91, 2014 
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Issue Date
Ablation Techniques/adverse effects* ; Acute Lung Injury/diagnostic imaging ; Acute Lung Injury/etiology* ; Acute Lung Injury/metabolism ; Acute Lung Injury/pathology ; Animals ; Collagen/metabolism ; Disease Models, Animal ; Male ; Mice ; Mice, Inbred C57BL ; Neoplasms/surgery ; Radiation Injuries, Experimental/diagnostic imaging ; Radiation Injuries, Experimental/etiology* ; Radiation Injuries, Experimental/metabolism ; Radiation Injuries, Experimental/pathology ; Radiosurgery/adverse effects* ; X-Ray Microtomography
In a previous study, we established an image-guided small-animal micro-irradiation system mimicking clinical stereotactic body radiotherapy (SBRT). The goal of this study was to develop a rodent model of acute phase lung injury after ablative irradiation. A radiation dose of 90 Gy was focally delivered to the left lung of C57BL/6 mice using a small animal stereotactic irradiator. At days 1, 3, 5, 7, 9, 11 and 14 after irradiation, the lungs were perfused with formalin for fixation and paraffin sections were stained with hematoxylin and eosin (H&E) and Masson's trichrome. At days 7 and 14 after irradiation, micro-computed tomography (CT) images of the lung were taken and lung functional measurements were performed with a flexiVent™ system. Gross morphological injury was evident 9 days after irradiation of normal lung tissues and dynamic sequential events occurring during the acute phase were validated by histopathological analysis. CT images of the mouse lungs indicated partial obstruction located in the peripheral area of the left lung. Significant alteration in inspiratory capacity and tissue damping were detected on day 14 after irradiation. An animal model of radiation-induced lung injury (RILI) in the acute phase reflecting clinical stereotactic body radiotherapy was established and validated with histopathological and functional analysis. This model enhances our understanding of the dynamic sequential events occurring in the acute phase of radiation-induced lung injury induced by ablative dose focal volume irradiation.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Park, Kwang Woo(박광우) ORCID logo https://orcid.org/0000-0002-9843-7985
Lee, Jung Il(이정일)
Lee, Ji Min(이지민)
Cho, Jae Ho(조재호) ORCID logo https://orcid.org/0000-0001-9966-5157
Choi, Won Hoon(최원훈)
Hong, Zhen-Yu(홍진우)
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