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Altered Vimentin Protein Expression in Human Dermal Microvascular Endothelial Cells After Ultraviolet or Intense Pulsed Light Treatment

 Jung U Shin  ;  Won Jai Lee  ;  Sang Ho Oh  ;  Do Young Kim  ;  Dae Suk Kim  ;  Inhee Jung  ;  Ju Hee Lee 
 Lasers in Surgery and Medicine, Vol.46(5) : 431-438, 2014 
Journal Title
 Lasers in Surgery and Medicine 
Issue Date
BACKGROUND:Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially N(ϵ) -(carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process. OBJECTIVES:To identify protein biomarkers of aging in skin. MATERIALS AND METHODS:Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting. RESULTS:Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment. CONCLUSION:Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.
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1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dae Suk(김대석)
Kim, Do Young(김도영) ORCID logo https://orcid.org/0000-0002-0194-9854
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
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