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Altered Vimentin Protein Expression in Human Dermal Microvascular Endothelial Cells After Ultraviolet or Intense Pulsed Light Treatment

DC Field Value Language
dc.contributor.author신정우-
dc.contributor.author오상호-
dc.contributor.author이원재-
dc.contributor.author이주희-
dc.contributor.author김대석-
dc.contributor.author김도영-
dc.date.accessioned2015-01-06T16:59:30Z-
dc.date.available2015-01-06T16:59:30Z-
dc.date.issued2014-
dc.identifier.issn0196-8092-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99162-
dc.description.abstractBACKGROUND:Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially N(ϵ) -(carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process. OBJECTIVES:To identify protein biomarkers of aging in skin. MATERIALS AND METHODS:Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting. RESULTS:Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment. CONCLUSION:Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.-
dc.description.statementOfResponsibilityopen-
dc.format.extent431~438-
dc.relation.isPartOfLASERS IN SURGERY AND MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiomarkers/metabolism-
dc.subject.MESHCells, Cultured-
dc.subject.MESHEndothelial Cells/metabolism-
dc.subject.MESHEndothelial Cells/radiation effects*-
dc.subject.MESHHumans-
dc.subject.MESHMicrovessels/cytology-
dc.subject.MESHProteomics-
dc.subject.MESHSkin Aging/physiology*-
dc.subject.MESHSkin Aging/radiation effects-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization-
dc.subject.MESHUltraviolet Rays*-
dc.subject.MESHUp-Regulation-
dc.subject.MESHVimentin/metabolism*-
dc.titleAltered Vimentin Protein Expression in Human Dermal Microvascular Endothelial Cells After Ultraviolet or Intense Pulsed Light Treatment-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Plastic Surgery & Reconstructive Surgery (성형외과학)-
dc.contributor.googleauthorJung U Shin-
dc.contributor.googleauthorWon Jai Lee-
dc.contributor.googleauthorSang Ho Oh-
dc.contributor.googleauthorDo Young Kim-
dc.contributor.googleauthorDae Suk Kim-
dc.contributor.googleauthorInhee Jung-
dc.contributor.googleauthorJu Hee Lee-
dc.identifier.doi10.1002/lsm.22253-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02149-
dc.contributor.localIdA02370-
dc.contributor.localIdA03005-
dc.contributor.localIdA00366-
dc.contributor.localIdA03171-
dc.contributor.localIdA00384-
dc.relation.journalcodeJ02159-
dc.identifier.eissn1096-9101-
dc.identifier.pmid24737666-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/lsm.22253/abstract-
dc.subject.keywordHDMEC-
dc.subject.keywordproteomics-
dc.subject.keywordskin aging-
dc.subject.keywordvimentin-
dc.contributor.alternativeNameShin, Jung U-
dc.contributor.alternativeNameOh, Sang Ho-
dc.contributor.alternativeNameLee, Won Jai-
dc.contributor.alternativeNameLee, Ju Hee-
dc.contributor.alternativeNameKim, Dae Suk-
dc.contributor.alternativeNameKim, Do Young-
dc.contributor.affiliatedAuthorShin, Jung U-
dc.contributor.affiliatedAuthorOh, Sang Ho-
dc.contributor.affiliatedAuthorLee, Won Jai-
dc.contributor.affiliatedAuthorKim, Dae Suk-
dc.contributor.affiliatedAuthorLee, Ju Hee-
dc.contributor.affiliatedAuthorKim, Do Young-
dc.rights.accessRightsfree-
dc.citation.volume46-
dc.citation.number5-
dc.citation.startPage431-
dc.citation.endPage438-
dc.identifier.bibliographicCitationLASERS IN SURGERY AND MEDICINE, Vol.46(5) : 431-438, 2014-
dc.identifier.rimsid51188-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers

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