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Human Leukocyte Antigen-G (HLA-G) Polymorphism and Expression in Breast Cancer Patients

 Seri Jeong  ;  Seho Park  ;  Byeong-Woo Park  ;  Younhee Park  ;  Oh-Joong Kwon  ;  Hyon-Suk Kim 
 PLOS ONE, Vol.9(5) : e98284, 2014 
Journal Title
Issue Date
Area Under Curve ; Base Sequence ; Breast Neoplasms/genetics* ; Breast Neoplasms/metabolism ; DNA Primers/genetics ; Enzyme-Linked Immunosorbent Assay ; Female ; Gene Expression Regulation, Neoplastic/genetics* ; HLA-G Antigens/genetics* ; HLA-G Antigens/metabolism* ; Humans ; Immunohistochemistry ; Logistic Models ; Molecular Sequence Data ; Neoplasm Metastasis/genetics* ; Polymorphism, Genetic* ; Sequence Analysis, DNA ; Tumor Escape/genetics*
Human leukocyte antigen-G (HLA-G) is known to be implicated in a tumor-driven immune escape mechanism in malignancies. The purpose of this study was to investigate HLA-G polymorphism and expression in breast cancer. HLA-G alleles were determined by direct DNA sequencing procedures from blood samples of 80 breast cancer patients and 80 healthy controls. Soluble HLA-G (sHLA-G) was measured by enzyme-linked immunosorbent assay (ELISA) from serum specimens. HLA-G expression in breast cancer lesions was also analyzed by immunohistochemistry staining. The presence of HLA-G 3′ untranslated region (UTR) 14-bp sequence was analyzed and found to be associated with reduced risk of breast cancer susceptibility based on HLA-G expression in tissues (P = 0.0407). Levels of sHLA-G were higher in the breast cancer group (median 117.2 U/mL) compared to the control group (median 10.1 U/mL, P<0.001). The area under the receiver operating characteristic curve (AU-ROC) values of sHLA-G for differentiating breast cancer from normal controls and for detecting metastasis from other stages of breast cancer were 0.89 and 0.79, respectively. HLA-G polymorphism and expression may be involved in breast carcinogenesis and sHLA-G concentrations could be used as a diagnostic marker for detecting breast cancer.
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Byeong Woo(박병우) ORCID logo https://orcid.org/0000-0003-1353-2607
Park, Se Ho(박세호) ORCID logo https://orcid.org/0000-0001-8089-2755
Jeong, Se Ri(정세리)
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