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Cited 3 times in

DA-6034 Induces [Ca2+]i Increase in Epithelial Cells

DC FieldValueLanguage
dc.contributor.author양유미-
dc.contributor.author지혜원-
dc.contributor.author김응권-
dc.contributor.author김태임-
dc.contributor.author박순홍-
dc.contributor.author신동민-
dc.date.accessioned2015-01-06T16:42:03Z-
dc.date.available2015-01-06T16:42:03Z-
dc.date.issued2014-
dc.identifier.issn1226-4512-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/98597-
dc.description.abstractDA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca2+ signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca2+ signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca2+-activated Cl- channels (CaCCs) and increased intracellular calcium concentrations ([Ca2+]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca2+]i in mouse salivary gland cells and human corneal epithelial cells. [Ca2+]i increase of DA-6034 was dependent on the Ca2+ entry from extracellular and Ca2+ release from internal Ca2+ stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca2+ stores. These results suggest that DA-6034 induces Ca2+ signaling via extracellular Ca2+ entry and RyRs-sensitive Ca2+ release from internal Ca2+ stores in epithelial cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent89~94-
dc.relation.isPartOfKOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleDA-6034 Induces [Ca2+]i Increase in Epithelial Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorYu-Mi Yang-
dc.contributor.googleauthorSoonhong Park-
dc.contributor.googleauthorHyeWon Ji-
dc.contributor.googleauthorTae-im Kim-
dc.contributor.googleauthorEung Kweon Kim-
dc.contributor.googleauthorKyung Koo Kang-
dc.contributor.googleauthorDong Min Shin-
dc.identifier.doi10.4196/kjpp.2014.18.2.89-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03974-
dc.contributor.localIdA00831-
dc.contributor.localIdA01080-
dc.contributor.localIdA01547-
dc.contributor.localIdA02091-
dc.contributor.localIdA05148-
dc.relation.journalcodeJ02104-
dc.identifier.eissn2093-3827-
dc.identifier.pmid24757369-
dc.subject.keywordCalcium signaling-
dc.subject.keywordDA-6034-
dc.subject.keywordEpithelial cells-
dc.subject.keywordEupatilin-
dc.contributor.alternativeNameYang, Yu-Mi-
dc.contributor.alternativeNameJi, Hye Won-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.contributor.alternativeNameKim, Tae Im-
dc.contributor.alternativeNamePark, Soon Hong-
dc.contributor.alternativeNameShin, Dong Min-
dc.contributor.affiliatedAuthorJi, Hye Won-
dc.contributor.affiliatedAuthorKim, Eung Kweon-
dc.contributor.affiliatedAuthorKim, Tae Im-
dc.contributor.affiliatedAuthorPark, Soon Hong-
dc.contributor.affiliatedAuthorShin, Dong Min-
dc.contributor.affiliatedAuthorYang, Yu Mi-
dc.citation.volume18-
dc.citation.number2-
dc.citation.startPage89-
dc.citation.endPage94-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.18(2) : 89-94, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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