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Viability of pulp stromal cells in cryopreserved deciduous teeth

 Eun Hye Ji  ;  Je Seon Song  ;  Seong-Oh Kim  ;  Mijeong Jeon  ;  Byung-Jai Choi  ;  Jae Ho Lee 
 CELL AND TISSUE BANKING, Vol.15(1) : 67-74, 2014 
Journal Title
Issue Date
Adolescent ; Apoptosis ; Cell Differentiation ; Cell Separation ; Cell Survival/physiology ; Cells, Cultured ; Child ; Child, Preschool ; Cryopreservation* ; Dental Pulp/cytology* ; Female ; Humans ; In Situ Nick-End Labeling ; Male ; Stem Cells/cytology* ; Stromal Cells/cytology* ; Tooth, Deciduous/cytology*
Cryopreservation ; Deciduous teeth ; Pulp stromal cell ; Cell viability
The cryopreservation of exfoliated deciduous teeth and harvesting of stem cells from them as required would reduce the costs and efforts associated with banking stem cells from primary teeth. The aim of this study was determine whether the viability of pulp stromal cells from deciduous teeth was influenced by the cryopreservation process itself or the period of cryopreservation. In total, 126 deciduous teeth were divided into three groups: (1) fresh, (2) cryopreserved for <3 months (cryo<3), and (3) cryopreserved for 3–9 months (cryo3–9). The viability of the pulp tissues was compared among the three groups by evaluating the outgrowth from pulp tissues and cell activity within those pulp tissues. In addition, the terminal deoxynucleotidyl transferase-mediated dUTP–biotin nick end labeling (TUNEL) assay was performed to compare cell apoptosis within fresh pulp tissue and pulp tissue that had been cryopreserved for 4 months. The outgrowth from and cell activity within the pulp tissues did not differ significantly between the fresh and cryo<3 pulp tissues. However, these parameters were significantly reduced in the cryo3–9 pulp tissue. In TUNEL assay, 4-month cryopreserved pulp tissues has more apoptotic cells than fresh group. In conclusion, it is possible to acquire pulp stromal cells from cryopreserved deciduous teeth. However, as the period of cryopreservation becomes longer, it is difficult to get pulp cells due to reduced cell viability.
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2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
Choi, Byung Jai(최병재)
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