Cited 11 times in
Time-dependent effect of combination therapy with erythropoietin and granulocyte colony-stimulating factor in a mouse model of hypoxic-ischemic brain injury
DC Field | Value | Language |
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dc.contributor.author | 서정화 | - |
dc.contributor.author | 유지혜 | - |
dc.contributor.author | 이종은 | - |
dc.contributor.author | 조성래 | - |
dc.contributor.author | 허지회 | - |
dc.date.accessioned | 2015-01-06T16:24:18Z | - |
dc.date.available | 2015-01-06T16:24:18Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1673-7067 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98042 | - |
dc.description.abstract | Erythropoietin (EPO) and granulocyte colony-stimulating factor (G-CSF) are likely to play broad roles in the brain. We investigated the effects of combination therapy with EPO and G-CSF in hypoxic-ischemic brain injury during the acute, subacute, and chronic phases. A total of 79 C57BL/6 mice with hypoxic-ischemic brain injury were randomly assigned acute (days 1-5), subacute (days 11-15) and chronic (days 28-32) groups. All of them were treated with G-CSF (250 μg/kg) and EPO (5000 U/kg) or saline daily for 5 consecutive days. Behavioral assessments and immunohistochemistry for angiogenesis, neurogenesis, and astrogliosis were performed with an 8-week follow-up. Hypoxia-inducible factor-1 (HIF-1) was also measured by Western blot analysis. The results showed that the combination therapy with EPO and G-CSF in the acute phase significantly improved rotarod performance and forelimb-use symmetry compared to the other groups, while subacute EPO and G-CSF therapy exhibited a modest improvement compared with the chronic saline controls. The acute treatment significantly increased the density of CD31(+) (PECAM-1) and α-smooth muscle actin(+) vessels in the frontal cortex and striatum, increased BrdU(+)/PSA-NCAM(+) neurogenesis in the subventricular zone, and decreased astroglial density in the striatum. Furthermore, acute treatment significantly increased the HIF-1 expression in the cytosol and nucleus, whereas chronic treatment did not change the HIF-1 expression, consistent with the behavioral outcomes. These results indicate that the induction of HIF-1 expression by combination therapy with EPO and G-CSF synergistically enhances not only behavioral function but also neurogenesis and angiogenesis while decreasing the astroglial response in a time-dependent manner. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 107~117 | - |
dc.relation.isPartOf | NEUROSCIENCE BULLETIN | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Brain/drug effects* | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Drug Therapy, Combination | - |
dc.subject.MESH | Erythropoietin/therapeutic use* | - |
dc.subject.MESH | Granulocyte Colony-Stimulating Factor/therapeutic use* | - |
dc.subject.MESH | Hypoxia-Inducible Factor 1, alpha Subunit/metabolism | - |
dc.subject.MESH | Hypoxia-Ischemia, Brain/drug therapy* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Motor Activity/drug effects | - |
dc.subject.MESH | Neurogenesis/drug effects | - |
dc.subject.MESH | Neuroprotective Agents/therapeutic use* | - |
dc.title | Time-dependent effect of combination therapy with erythropoietin and granulocyte colony-stimulating factor in a mouse model of hypoxic-ischemic brain injury | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Ji Hea Yu | - |
dc.contributor.googleauthor | Jung Hwa Seo | - |
dc.contributor.googleauthor | Jong Eun Lee | - |
dc.contributor.googleauthor | Ji Hoe Heo | - |
dc.contributor.googleauthor | Sung-Rae Cho | - |
dc.identifier.doi | 10.1007/s12264-013-1397-9 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01906 | - |
dc.contributor.localId | A02521 | - |
dc.contributor.localId | A03831 | - |
dc.contributor.localId | A04369 | - |
dc.contributor.localId | A03146 | - |
dc.relation.journalcode | J02363 | - |
dc.identifier.eissn | 1995-8218 | - |
dc.identifier.pmid | 24435306 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs12264-013-1397-9 | - |
dc.subject.keyword | erythropoietin | - |
dc.subject.keyword | granulocyte colonystimulating factor | - |
dc.subject.keyword | hypoxia-inducible factor-1 | - |
dc.subject.keyword | hypoxicischemic brain injury | - |
dc.contributor.alternativeName | Seo, Jung Hwa | - |
dc.contributor.alternativeName | Yu, Ji Hea | - |
dc.contributor.alternativeName | Lee, Jong Eun | - |
dc.contributor.alternativeName | Cho, Sung Rae | - |
dc.contributor.alternativeName | Heo, Ji Hoe | - |
dc.contributor.affiliatedAuthor | Seo, Jung Hwa | - |
dc.contributor.affiliatedAuthor | Yu, Ji Hea | - |
dc.contributor.affiliatedAuthor | Cho, Sung Rae | - |
dc.contributor.affiliatedAuthor | Heo, Ji Hoe | - |
dc.contributor.affiliatedAuthor | Lee, Jong Eun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 30 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 107 | - |
dc.citation.endPage | 117 | - |
dc.identifier.bibliographicCitation | NEUROSCIENCE BULLETIN, Vol.30(1) : 107-117, 2014 | - |
dc.identifier.rimsid | 54349 | - |
dc.type.rims | ART | - |
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