PDiabetic neuropathy is one of the most common diabetes related complications including diabetic nephropathy and retinopahty. In clinical practices, nerve conduction velocity (NCV) has been used as a standard method for diagnosing diabetic neuropathy. However, it applies maximum current of 100mA to nerves causing stress and pain to patients. In this study, as a non-invasive method, TcpO₂ was utilized to investigate the difference and relationship between TcpO₂ and SpO₂ of normal and diabetic neuropathy subjects. In addition, a new method of diagnosing diabetic neuropathy using TcpO₂ is suggested. 50 normal subjects and 50 diabetic patients with neuropathy diagnosed by NCV participated in this study. Parameters used in this study were TcpO₂, TcpCO₂, and SpO₂. As a result of the TcpO₂ measurements, statistical significances were found from TcpO₂ of hands and feet from normal and patients group(p<0.01). SpO₂ measured from index finger of normal and patient groups showed no statistical significance(p>0.05). On the other hand, SpO₂ measured from great toes of normal and patient groups showed statistical significance(p<0.01). Correlation coefficient between SpO₂ of finger and TcpO₂ of hand was 0.400 (p<0.01) and SpO₂ of toe and TcpO₂ of foot was 0.471(p<0.01). Both correlation values were statistically significant. Sensitivities and specificities of the TcpO₂ method were found to be 66 % and 92 %, respectively. If the suggested TcpO₂ method is used periodically, prevention and early diagnosis of diabetic neuropathy would be possible.