Purpose: To evaluate the factors affecting treatment outcome of children with anisometropic amblyopia. Methods: We retrospectively investigated the treatment outcome of 57 children who were diagnosed as anisometropic amblyopia. The age at initial treatment, initial best corrected visual acuity of the amblyopic eye, amount and type of anisometropia, strabismus and compliance of treatment were investigated. Results: The mean age and standard deviation at initial treatment was 5.3±1.8(3-9) years. The absolute average values of spherical and cylindrical anisometropia were 2.87±1.99D and 1.87±2.24D respectively. While compliance was significantly related to treatment outcome, the age at initial treatment, visual acuity on amblyopic eye, amount and type of anisometropia, and strabismus had no significant influence on treatment outcome. Conclusions: Treatment compliance was the most significant factor in the treatment of anisometropic amblyopia.