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ERK 1/2 activation in enhanced osteogenesis of human mesenchymal stem cells in poly(lactic-glycolic acid) by cyclic hydrostatic pressure

Authors
 Su-Hyang Kim  ;  Yon Rak Choi  ;  Jin Woo Lee  ;  Sung-Jae Kim  ;  Ki Dong Park  ;  Jung Woog Shin  ;  Min Sung Park 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.80A(4) : 826-836, 2007 
Journal Title
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 
ISSN
 1549-3296 
Issue Date
2007
MeSH
Cell Culture Techniques ; Cells, Cultured ; Humans ; Hydrostatic Pressure ; Mechanotransduction, Cellular* ; Mesenchymal Stem Cells/enzymology* ; Mitogen-Activated Protein Kinase 1/metabolism* ; Mitogen-Activated Protein Kinase 3/metabolism* ; Osteogenesis* ; Stress, Mechanical
Keywords
mesenchymal stem cell ; 3D culture ; hydrostatic pressure and fluid flow ; osteogenesis ; mechanotransducer ; ERK1/2 activation
Abstract
The aim of this study was to identify the signal transduction pathways and mechano-transducers that play critical roles in the processes induced by changes in cyclic hydrostatic pressure and fluid shear in 3-dimensional (3D) culture systems. Mesenchymal stem cells were loaded into a polymeric scaffold and divided into three groups according to the stress treatment: static, fluid shear, and hydrostatic pressure with fluid shear. Cells were exposed daily to a hydrostatic pressure of 0.2 MPa for 1 min followed by 14 min rest with fluid flow at 30 rpm. Protein extracts were analyzed by Western blot for extracellular signal-regulated kinase 1/2 (ERK1/2). The complexes were cultured under the mechanical stimuli for 21 days with or without phospho-ERK1/2 inhibitor (U0126) and evaluated by RT-PCR, calcium contents, and immunohistochemistry. Under conditions of mechanical stimulation, the activation of ERK1/2 was sustained or increased with time. U0126 suppressed mechanical stimuli-induced expression of osteocalcin. In addition, calcium contents and the degrees of osteocalcin and osteopontin staining were decreased by this inhibitor. These results demonstrate that mechanical stimuli, particularly hydrostatic pressure with fluid shear, enhance osteogenesis in 3D culture systems via ERK1/2 activation.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.30945/abstract
DOI
10.1002/jbm.a.30945
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Sung Jae(김성재)
Kim, Su Hyang(김수향)
Park, Min Sung(박민성)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Choi, Yon Rak(최연락)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96231
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