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Sequence Polymorphisms of Major German Cockroach Allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5

Authors
 Kyoung Yong Jeong  ;  Haeseok Lee  ;  Tai-Soon Yong  ;  Chein-Soo Hong  ;  Kyoung-jin Jeong  ;  Myung-hee Yi  ;  Kwang Hyun Shin 
Citation
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.145(1) : 1-8, 2007 
Journal Title
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 
ISSN
 1018-2438 
Issue Date
2007
MeSH
Allergens/genetics* ; Amino Acid Sequence ; Animals ; Cockroaches/genetics* ; Cockroaches/immunology ; DNA, Complementary/genetics ; Female ; Insect Proteins/genetics* ; Male ; Molecular Sequence Data ; Polymorphism, Genetic ; Sequence Alignment ; Sequence Analysis, DNA
Abstract
BACKGROUND: The allergenicity of allergens could be influenced by amino acid substitutions in B- or T-cell epitope regions. The German cockroach is known to produce potent allergens inducing strong IgE-mediated allergic reactions. This study was performed to investigate sequence variations in major allergens of the German cockroach. METHODS: Reverse transcriptase PCR was used to amplify the cDNA sequences encoding major allergens of the German cockroach (Bla g 1, Bla g 2, Bla g 4, and Bla g 5). RESULTS: The deduced amino acid sequences revealed 38 Bla g 1 variants with 1-7 amino acid substitutions (98.6-99.8% identity), 28 Bla g 2 variants with 1-3 substitutions (99.1-99.7%), 27 Bla g 4 variants with 0-32 substitutions (82.4-100%), and 8 Bla g 5 variants with 1-2 substitutions (99.0-99.5%), respectively. Bla g 1 and Bla g 2 showed sporadic amino acid substitutions despite the divergence in their sequences. Bla g 4 exhibited frequent variations, with clusters of substitutions in residues 29-38, 52-80, and 132-155. Sequence variations in Bla g 4 imply the presence of multiple isoforms and isoallergens, which may in turn have various effects on the IgE-binding capacity and T-cell responsiveness. Only 8 variants were found in Bla g 5, with infrequent amino acid changes of one or two residues. CONCLUSIONS: Analyses of T-cell and IgE-binding epitope regions would clarify the effect of sequence polymorphisms on allergenicity, which in turn will aid in the design of allergen formulations for diagnosis and immunotherapy for cockroach allergies.
Full Text
http://www.karger.com/Article/FullText/107460
DOI
10.1159/000107460
Appears in Collections:
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Lee, Hae Seok(이해석)
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96126
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