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Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity

 Soo-Young Choi  ;  Jung-Ho Sohn  ;  Jung-Won Park  ;  Chein-Soo Hong  ;  Eun-Kyung Lee  ;  Yong-Won Lee 
 International Archives of Allergy and Immunology, Vol.144(4) : 267-274, 2007 
Journal Title
 International Archives of Allergy and Immunology 
Issue Date
Adolescent ; Adult ; Allergens/adverse effects* ; Allergens/genetics ; Allergens/immunology ; Amino Acid Sequence ; Antigens, Plant/adverse effects* ; Antigens, Plant/genetics ; Antigens, Plant/immunology ; Child ; DNA, Complementary ; Double-Blind Method ; Fagopyrum/chemistry ; Fagopyrum/immunology* ; Female ; Food Hypersensitivity/diagnosis ; Food Hypersensitivity/immunology ; Humans ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Male ; Molecular Sequence Data ; Plant Extracts/adverse effects ; Plant Extracts/immunology ; Plant Proteins/adverse effects* ; Plant Proteins/genetics ; Plant Proteins/immunology ; Recombinant Proteins/immunology ; Skin Tests
BACKGROUND: The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. METHODS: cDNA of the 19-kD BW allergen was cloned and expressed in Escherichia coli. Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. RESULTS: The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew (Ana o 1), English walnut (Jug r 2) and 7 S globulin from Sesamum indicum. The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839-1.012), p < 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725-0.995), p <0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661-0.945), p = 0.002]. CONCLUSIONS: The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Sohn, Jung Ho(손정호)
Lee, Yong Won(이용원)
Lee, Eun Kyung(이은경)
Choi, Soo Young(최수영)
Hong, Chein Soo(홍천수)
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