Cited 21 times in
Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박중원 | - |
dc.contributor.author | 손정호 | - |
dc.contributor.author | 이용원 | - |
dc.contributor.author | 이은경 | - |
dc.contributor.author | 최수영 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2014-12-21T16:40:02Z | - |
dc.date.available | 2014-12-21T16:40:02Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96121 | - |
dc.description.abstract | BACKGROUND: The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. METHODS: cDNA of the 19-kD BW allergen was cloned and expressed in Escherichia coli. Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. RESULTS: The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew (Ana o 1), English walnut (Jug r 2) and 7 S globulin from Sesamum indicum. The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839-1.012), p < 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725-0.995), p <0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661-0.945), p = 0.002]. CONCLUSIONS: The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 267~274 | - |
dc.relation.isPartOf | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Allergens/adverse effects* | - |
dc.subject.MESH | Allergens/genetics | - |
dc.subject.MESH | Allergens/immunology | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Antigens, Plant/adverse effects* | - |
dc.subject.MESH | Antigens, Plant/genetics | - |
dc.subject.MESH | Antigens, Plant/immunology | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | DNA, Complementary | - |
dc.subject.MESH | Double-Blind Method | - |
dc.subject.MESH | Fagopyrum/chemistry | - |
dc.subject.MESH | Fagopyrum/immunology* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Food Hypersensitivity/diagnosis | - |
dc.subject.MESH | Food Hypersensitivity/immunology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin E/blood | - |
dc.subject.MESH | Immunoglobulin E/immunology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Plant Extracts/adverse effects | - |
dc.subject.MESH | Plant Extracts/immunology | - |
dc.subject.MESH | Plant Proteins/adverse effects* | - |
dc.subject.MESH | Plant Proteins/genetics | - |
dc.subject.MESH | Plant Proteins/immunology | - |
dc.subject.MESH | Recombinant Proteins/immunology | - |
dc.subject.MESH | Skin Tests | - |
dc.title | Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Soo-Young Choi | - |
dc.contributor.googleauthor | Jung-Ho Sohn | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Eun-Kyung Lee | - |
dc.contributor.googleauthor | Yong-Won Lee | - |
dc.identifier.doi | 10.1159/000106315 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A01993 | - |
dc.contributor.localId | A02981 | - |
dc.contributor.localId | A03033 | - |
dc.contributor.localId | A04093 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J01074 | - |
dc.identifier.eissn | 1423-0097 | - |
dc.identifier.pmid | 17641547 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/106315 | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Sohn, Jung Ho | - |
dc.contributor.alternativeName | Lee, Yong Won | - |
dc.contributor.alternativeName | Lee, Eun Kyung | - |
dc.contributor.alternativeName | Choi, Soo Young | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Sohn, Jung Ho | - |
dc.contributor.affiliatedAuthor | Lee, Yong Won | - |
dc.contributor.affiliatedAuthor | Lee, Eun Kyung | - |
dc.contributor.affiliatedAuthor | Choi, Soo Young | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 144 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 267 | - |
dc.citation.endPage | 274 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.144(4) : 267-274, 2007 | - |
dc.identifier.rimsid | 35447 | - |
dc.type.rims | ART | - |
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