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Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity

DC FieldValueLanguage
dc.contributor.author박중원-
dc.contributor.author손정호-
dc.contributor.author이용원-
dc.contributor.author이은경-
dc.contributor.author최수영-
dc.contributor.author홍천수-
dc.date.accessioned2014-12-21T16:40:02Z-
dc.date.available2014-12-21T16:40:02Z-
dc.date.issued2007-
dc.identifier.issn1018-2438-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/96121-
dc.description.abstractBACKGROUND: The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. METHODS: cDNA of the 19-kD BW allergen was cloned and expressed in Escherichia coli. Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. RESULTS: The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew (Ana o 1), English walnut (Jug r 2) and 7 S globulin from Sesamum indicum. The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839-1.012), p < 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725-0.995), p <0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661-0.945), p = 0.002]. CONCLUSIONS: The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract.-
dc.description.statementOfResponsibilityopen-
dc.format.extent267~274-
dc.relation.isPartOfInternational Archives of Allergy and Immunology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleCharacterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSoo-Young Choi-
dc.contributor.googleauthorJung-Ho Sohn-
dc.contributor.googleauthorJung-Won Park-
dc.contributor.googleauthorChein-Soo Hong-
dc.contributor.googleauthorEun-Kyung Lee-
dc.contributor.googleauthorYong-Won Lee-
dc.identifier.doi10.1159/000106315-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01681-
dc.contributor.localIdA01993-
dc.contributor.localIdA02981-
dc.contributor.localIdA03033-
dc.contributor.localIdA04093-
dc.contributor.localIdA04448-1-
dc.relation.journalcodeJ01074-
dc.identifier.urlhttp://www.karger.com/Article/FullText/106315-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameSohn, Jung Ho-
dc.contributor.alternativeNameLee, Yong Won-
dc.contributor.alternativeNameLee, Eun Kyung-
dc.contributor.alternativeNameChoi, Soo Young-
dc.contributor.alternativeNameHong, Chein Soo-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorSohn, Jung Ho-
dc.contributor.affiliatedAuthorLee, Yong Won-
dc.contributor.affiliatedAuthorLee, Eun Kyung-
dc.contributor.affiliatedAuthorChoi, Soo Young-
dc.contributor.affiliatedAuthorHong, Chein Soo-
dc.rights.accessRightsnot free-
dc.citation.volume144-
dc.citation.number4-
dc.citation.startPage267-
dc.citation.endPage274-
dc.identifier.bibliographicCitationInternational Archives of Allergy and Immunology, Vol.144(4) : 267-274, 2007-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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