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Pharmacokinetics of GST-TatdMt, a recombinant fusion protein possessing potent anti-obesity activity, in Mice

Authors
 Beom Soo Shin  ;  Jin Hyuk Seo  ;  Sun Dong Yoo  ;  Min-Nyung Lee  ;  Man-Wook Hur 
Citation
 ARCHIVES OF PHARMACAL RESEARCH, Vol.30(9) : 1162-1167, 2007 
Journal Title
 ARCHIVES OF PHARMACAL RESEARCH 
ISSN
 0253-6269 
Issue Date
2007
Abstract
This study examined the absorption and pharmacokinetic disposition of125l-GST-TatdMt, a recombinant Tat protein possessing potent anti-obesity activity, in mice after vascular and extravascular administration. GST-TatdMt was over-expressed in E. coli, purified, and radio-iodinated using the IODO-GEN method.125I-GST-TatdMt was administered to mice by i.v., i.p. and oral administration at doses of 652.7 nCi (102.3 μg). Upon i.v. injection, the average terminal elimination half-life (t1/2,λz), AUC and AUMC were 6.4 h, 318.2 nCi·h/mL and 2518 nCi·h2/ mL, respectively. The highest radioactivity was observed in lung followed by liver, spleen, heart and kidney. The t1/2,λz values obtained from i.v., i.p., and oral administration were comparable from each other (range 5.8–6.4 h). The absolute bioavailability of125I-GST-TatdMt was 42.8% and 60.5% after p.o. and i.p. administration, respectively. Given the cell-penetrating nature,125l-GST-TatdMt may be absorbed into the systemic circulation to a relatively high extent after extravascular administration.
Full Text
http://link.springer.com/article/10.1007/BF02980253
DOI
10.1007/BF02980253
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95660
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