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Identification of antigenic proteins in Trichomonas vaginalis

Authors
 Hye-Yeon Lee  ;  Sujin Hyung  ;  Jong Woong Lee  ;  Juri Kim  ;  Myeong Heon Shin  ;  Jae-Sook Ryu  ;  Soon-Jung Park 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.49(1) : 79-83, 2011 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2011
MeSH
Animals ; Antigens, Protozoan/genetics ; Antigens, Protozoan/immunology* ; Female ; Humans ; Molecular Sequence Data ; Protozoan Proteins/genetics ; Protozoan Proteins/immunology* ; Rats ; Trichomonas Infections/parasitology ; Trichomonas vaginalis/genetics ; Trichomonas vaginalis/immunology*
Keywords
Trichomonas vaginalis ; antigenic protein ; membrane protein ; immunoscreening
Abstract
Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage λ Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, α-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.
Files in This Item:
T201194071.pdf Download
DOI
10.3347/kjp.2011.49.1.79
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Hye Yeon(이혜연)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95361
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