2 82

Cited 8 times in

Cyclosporine A induces nerve growth factor expression via activation of MAPK p38 and NFAT5

Authors
 Lee, Joon H ; Kim, Jee Won ; Lee, Hyung Keun ; Seong, Gong Je ; Im, Young Sun 
Citation
 Cornea, Vol.30(Suppl 1) : s19-s24, 2011 
Journal Title
 Cornea 
ISSN
 0277-3740 
Issue Date
2011
Abstract
PURPOSE: We investigated the effects of cyclosporine A (CsA) on the mechanism of nerve growth factor (NGF) expression using a cultured human corneal epithelial cell line (HCECL). METHODS: NGF transcription and production levels were assessed after treatment of cells with various concentrations of CsA. Activities of mitogen-activated protein kinase (MAPK), nuclear factor Kappa B (NF-κB), activator protein-1 (AP-1), and nuclear factor of activated T cells (NFATs) influenced by CsA were determined using a luciferase assay. The translocation activity of NFAT5 was assessed by confocal microscopy and Western immunoblotting after CsA treatment. Transcriptional activity of NGF was measured after pretreatment of cells with SB20429 (a p38 inhibitor) and NFAT5 small interfering RNA. RESULTS: NGF was induced after treatment with CsA, but not dexamethasone, in the HCECL. NGF expression was mediated via p38 phosphorylation and NFAT5 activation. Transcriptional activities of NF-κB, AP-1, and NFAT1 were not stimulated by CsA; however, nuclear translocation of NFAT5 was markedly upregulated by CsA. CsA-induced NGF production was markedly decreased on inhibition of NFAT5 or SB20429. CONCLUSIONS: CsA is a potent inducer of NGF in the HCECL. These results suggest that CsA mediates NGF expression through activation of p38 and NFAT5.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/95184
DOI
10.1097/ICO.0b013e3182281028
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Medical Research Center
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Ophthalmology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00003226-201110001-00005&LSLINK=80&D=ovft
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse