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Immunotherapy of malignant melanoma with tumor lysate-pulsed autologous monocyte-derived dendritic cells

 Dae Suk Kim  ;  Dong Hyun Kim  ;  Boncheol Goo  ;  Young Hun Cho  ;  Jin Mo Park  ;  Tae Hyung Lee  ;  Hyun Ok Kim  ;  Han-Soo Kim  ;  Hyunah Lee  ;  Jong Doo Lee  ;  Dashlkhumbe Byamba  ;  Jeong Hwan Je  ;  Min-Geol Lee 
 YONSEI MEDICAL JOURNAL, Vol.52(6) : 990-998, 2011 
Journal Title
Issue Date
Dendritic Cells/cytology* ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Humans ; Immunotherapy/methods* ; Melanoma/therapy* ; Monocytes/cytology* ; Treatment Outcome
Dendritic cell ; immunotherapy ; malignant melanoma
PURPOSE: Dendritic cell (DC) vaccination for melanoma was introduced because melanoma carries distinct tumor-associated antigens. The purpose of this study was to investigate the efficacy and safety of DC vaccination for melanoma in Korea.

MATERIALS AND METHODS: Five patients with stage IV and one with stage II were enrolled. Autologous monocyte-derived DCs (MoDCs) were cultured and pulsed with tumor-lysate, keyhole limpet hemocyanin, and cytokine cocktail for mature antigen-loaded DC. DC vaccination was repeated four times at 2-week intervals and 2-4×10⁷ DC were injected each time.

RESULTS: Reduced tumor volume was observed by PET-CT in three patients after DC vaccination. Delayed type hypersensitivity responses against tumor antigen were induced in five patients. Tumor antigen-specific IFN-γ-producing peripheral blood mononuclear cells were detected with enzyme-linked immunosorbent spot in two patients. However, the overall clinical outcome showed disease progression in all patients.

CONCLUSION: In this study, DC vaccination using tumor antigen-loaded, mature MoDCs led to tumor regression in individual melanoma patients. Further standardization of DC vaccination protocol is required to determine which parameters lead to better anti-tumor responses and clinical outcomes.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Han Soo(김한수)
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Lee, Min Geol(이민걸) ORCID logo https://orcid.org/0000-0001-7040-5335
Lee, Jong Doo(이종두)
Je, Jeong Hwan(제정환)
Cho, Young Hun(조영훈)
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