3 667

Cited 7 times in

Augmentation of rat skin flap viability by relaxin-expressing adenovirus

Authors
 Won Jai Lee  ;  Chae-Ok Yun  ;  In Sik Yun  ;  Yong-Oock Kim  ;  Il-Kyu Choi  ;  Tae Jin Yun  ;  Dong Kyun Rah 
Citation
 WOUND REPAIR AND REGENERATION, Vol.19(6) : 709-717, 2011 
Journal Title
WOUND REPAIR AND REGENERATION
ISSN
 1067-1927 
Issue Date
2011
MeSH
Adenoviridae ; Angiogenesis Inhibitors/pharmacology ; Animals ; Antibodies, Monoclonal, Humanized/pharmacology ; Bevacizumab ; Fluorescent Antibody Technique ; Genetic Vectors ; Graft Survival* ; Male ; Microcirculation ; Rats ; Rats, Sprague-Dawley ; Relaxin/pharmacology* ; Skin/blood supply ; Skin/metabolism ; Surgical Flaps*/blood supply ; Vascular Endothelial Growth Factor A/immunology ; Vascular Endothelial Growth Factor A/metabolism
Abstract
Relaxin (RLX) has multiple vascular actions, including vasodilation and angiogenesis, which occur via induction of vascular endothelial growth factor (VEGF) expression. We generated a RLX-expressing (dE1-RGD/lacZ/RLX) adenovirus and investigated whether it enhances skin flap survival. Thirty Sprague-Dawley rats were divided into three groups: RLX-expressing adenovirus group, control virus group, and phosphate-buffered saline (PBS) group. Two days before surgery and immediately after flap elevation, the caudally based flap that was 3 × 9 cm in size was subdermally injected with the dE1-RGD/lacZ/RLX virus (10⁷ PFU), dE1-RGD/lacZ virus (10⁷ PFU), or PBS. The surviving area of the flap and the amount of blood flow were measured. On postoperative day 10, CD31-positive vessels and VEGF protein expression were examined. We observed a significant increase in the survival area of the flap in the RLX group. Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. CD31-positive vessels and VEGF protein expression were significantly greater in the RLX group. Thus, administration of RLX-expressing adenovirus into elevated skin flaps increased VEGF expression, the number of capillaries, and blood flow to the flap, thereby improving skin flap survival.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1524-475X.2011.00733.x/abstract
DOI
10.1111/j.1524-475X.2011.00733.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yong Oock(김용욱) ORCID logo https://orcid.org/0000-0002-3756-4809
Rah, Dong Kyun(나동균)
Yun, In Sik(윤인식) ORCID logo https://orcid.org/0000-0003-1103-7047
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94733
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links