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Augmentation of rat skin flap viability by relaxin-expressing adenovirus

Authors
 Won Jai Lee ; Chae-Ok Yun ; Dong Kyun Rah ; Tae Jin Yun ; Il-Kyu Choi ; Yong-Oock Kim ; In Sik Yun 
Citation
 Wound Repair and Regeneration, Vol.19(6) : 709~717, 2011 
Journal Title
 Wound Repair and Regeneration 
ISSN
 1067-1927 
Issue Date
2011
Abstract
Relaxin (RLX) has multiple vascular actions, including vasodilation and angiogenesis, which occur via induction of vascular endothelial growth factor (VEGF) expression. We generated a RLX-expressing (dE1-RGD/lacZ/RLX) adenovirus and investigated whether it enhances skin flap survival. Thirty Sprague-Dawley rats were divided into three groups: RLX-expressing adenovirus group, control virus group, and phosphate-buffered saline (PBS) group. Two days before surgery and immediately after flap elevation, the caudally based flap that was 3 × 9 cm in size was subdermally injected with the dE1-RGD/lacZ/RLX virus (10⁷ PFU), dE1-RGD/lacZ virus (10⁷ PFU), or PBS. The surviving area of the flap and the amount of blood flow were measured. On postoperative day 10, CD31-positive vessels and VEGF protein expression were examined. We observed a significant increase in the survival area of the flap in the RLX group. Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. CD31-positive vessels and VEGF protein expression were significantly greater in the RLX group. Thus, administration of RLX-expressing adenovirus into elevated skin flaps increased VEGF expression, the number of capillaries, and blood flow to the flap, thereby improving skin flap survival.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94733
DOI
10.1111/j.1524-475X.2011.00733.x
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Plastic Surgery & Reconstructive Surgery
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Link
 http://onlinelibrary.wiley.com/doi/10.1111/j.1524-475X.2011.00733.x/abstract
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