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Augmentation of rat skin flap viability by relaxin-expressing adenovirus

 Won Jai Lee  ;  Chae-Ok Yun  ;  Dong Kyun Rah  ;  Tae Jin Yun  ;  Il-Kyu Choi  ;  Yong-Oock Kim  ;  In Sik Yun 
 Wound Repair and Regeneration, Vol.19(6) : 709-717, 2011 
Journal Title
 Wound Repair and Regeneration 
Issue Date
Relaxin (RLX) has multiple vascular actions, including vasodilation and angiogenesis, which occur via induction of vascular endothelial growth factor (VEGF) expression. We generated a RLX-expressing (dE1-RGD/lacZ/RLX) adenovirus and investigated whether it enhances skin flap survival. Thirty Sprague-Dawley rats were divided into three groups: RLX-expressing adenovirus group, control virus group, and phosphate-buffered saline (PBS) group. Two days before surgery and immediately after flap elevation, the caudally based flap that was 3 × 9 cm in size was subdermally injected with the dE1-RGD/lacZ/RLX virus (10⁷ PFU), dE1-RGD/lacZ virus (10⁷ PFU), or PBS. The surviving area of the flap and the amount of blood flow were measured. On postoperative day 10, CD31-positive vessels and VEGF protein expression were examined. We observed a significant increase in the survival area of the flap in the RLX group. Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. CD31-positive vessels and VEGF protein expression were significantly greater in the RLX group. Thus, administration of RLX-expressing adenovirus into elevated skin flaps increased VEGF expression, the number of capillaries, and blood flow to the flap, thereby improving skin flap survival.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Plastic Surgery & Reconstructive Surgery (성형외과학교실)
Yonsei Authors
김용욱(Kim, Yong Oock) ; 나동균(Rah, Dong Kyun) ; 윤인식(Yun, In Sik) ; 이원재(Lee, Won Jai)
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