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복구효소를 이용한 손상된 DNA의 STR 유전자형 분석

Other Titles
 Application of a Repair Enzyme to STR Analysis of Damaged DNA 
Authors
 심정은  ;  이환영  ;  양우익  ;  이승환  ;  신경진 
Citation
 Korean Journal of Legal Medicine (대한법의학회지), Vol.35(2) : 114-119, 2011 
Journal Title
 Korean Journal of Legal Medicine  (대한법의학회지) 
ISSN
 1225-0589 
Issue Date
2011
Abstract
DNA found at the scene of a crime is often damaged and degraded to small fragments and remained in low quantity. Therefore, there are some difficulties in forensic STR typing of these DNA samples. In this study, to overcome these limitations as different approach, we applied repairing enzyme to damaged DNA. The efficacy of a repair enzyme system (PreCR Repair Mix) was evaluated by using several types of artificially damaged and naturally damaged DNA. The results showed that autosomal STR amplification produced increased yield in the DNA damaged by UV irradiation, oxidation, or acid/heat, and DNA from dried blood spot and dried saliva spot by treatment of a repair enzyme, but not in DNA extracted from old skeletal remains. In conclusion, a repair enzyme will be efficiently applied to forensic samples which were damaged by UV irradiation, oxidation, and acid/heat
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94732
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과)
Yonsei Authors
신경진(Shin, Kyoung Jin) ; 심정은(Sim, Jeong Eun) ; 양우익(Yang, Woo Ick) ; 이환영(Lee, Hwan Young)
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