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Correlations of 3T DCE-MRI quantitative parameters with microvessel density in a human-colorectal-cancer xenograft mouse model.

Authors
 Sung Jun Ahn  ;  Chan Sik An  ;  Woong Sub Koom  ;  Ho-Taek Song  ;  Jin-Suck Suh 
Citation
 KOREAN JOURNAL OF RADIOLOGY, Vol.12(6) : 722-730, 2011 
Journal Title
KOREAN JOURNAL OF RADIOLOGY
ISSN
 1229-6929 
Issue Date
2011
MeSH
Animals ; Capillary Permeability ; Colorectal Neoplasms/blood supply* ; Colorectal Neoplasms/pathology ; Contrast Media* ; Female ; Gadolinium ; Humans ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Magnetic Resonance Imaging* ; Mice ; Mice, Nude ; Microvessels/pathology* ; Neoplasm Transplantation ; Neovascularization, Pathologic/diagnosis
Keywords
Angiogenesis ; Colorectal cancer ; DLD-1 ; Ktrans ; MVD ; Magnetic resonance imaging ; Nude mouse model ; Permeability ; T1 dynamic contrast enhanced imaging
Abstract
OBJECTIVE: To investigate the correlation between quantitative dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) parameters and microvascular density (MVD) in a human-colon-cancer xenograft mouse model using 3 Tesla MRI.

MATERIALS AND METHODS: A human-colon-cancer xenograft model was produced by subcutaneously inoculating 1 × 10(6) DLD-1 human-colon-cancer cells into the right hind limbs of 10 mice. The tumors were allowed to grow for two weeks and then assessed using MRI. DCE-MRI was performed by tail vein injection of 0.3 mmol/kg of gadolinium. A region of interest (ROI) was drawn at the midpoints along the z-axes of the tumors, and a Tofts model analysis was performed. The quantitative parameters (K(trans), K(ep) and V(e)) from the whole transverse ROI and the hotspot ROI of the tumor were calculated. Immunohistochemical microvessel staining was performed and analyzed according to Weidner's criteria at the corresponding MRI sections. Additional Hematoxylin and Eosin staining was performed to evaluate tumor necrosis. The Mann-Whitney test and Spearman's rho correlation analysis were performed to prove the existence of a correlation between the quantitative parameters, necrosis, and MVD.

RESULTS: Whole transverse ROI of the tumor showed no significant relationship between the MVD values and quantitative DCE-MRI parameters. In the hotspot ROI, there was a difference in MVD between low and high group of K(trans) and K(ep) that had marginally statistical significance (ps = 0.06 and 0.07, respectively). Also, K(trans) and K(ep) were found to have an inverse relationship with MVD (r = -0.61, p = 0.06 in K(trans); r = -0.60, p = 0.07 in K(ep)).

CONCLUSION: Quantitative analysis of T1-weighted DCE-MRI using hotspot ROI may provide a better histologic match than whole transverse section ROI. Within the hotspots, K(trans) and K(ep) tend to have a reverse correlation with MVD in this colon cancer mouse model.
Files in This Item:
T201104008.pdf Download
DOI
10.3348/kjr.2011.12.6.722
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Koom, Woong Sub(금웅섭) ORCID logo https://orcid.org/0000-0002-9435-7750
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Song, Ho Taek(송호택) ORCID logo https://orcid.org/0000-0002-6655-2575
Ahn, Sung Jun(안성준) ORCID logo https://orcid.org/0000-0003-0075-2432
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94598
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