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Evaluation of automated serum des-gamma-carboxyprothrombin (DCP) assays for detecting hepatocellular carcinoma

Authors
 Jonghyeon Choi ; Yongjung Park ; Hyon-Suk Kim ; Jeong-Ho Kim 
Citation
 Clinical Biochemistry, Vol.44(17-18) : 1464~1468, 2011 
Journal Title
 Clinical Biochemistry 
ISSN
 0009-9120 
Issue Date
2011
Abstract
OBJECTIVES: We evaluated two new autoanalyzers, μTAS and Lumipulse for des-γ-carboxyprothrombin (DCP) assay. MATERIAL AND METHODS: Analytical performance was evaluated, and the upper reference limit of the 97.5th percentile for DCP was re-established using sera from 140 healthy individuals. DCP levels were determined by the two autoanalyzers and EIA in a total of 239 sera from HCC patients (n=120) and those without HCC (n=119). RESULTS: Total imprecision of the two automated assays was <5% CV. Analytical measurement ranges (AMRs) were verified to be linear. The new reference limits were 29.5 mAU/mL for μTAS and 35.0 mAU/mL for Lumipulse. There were proportional and constant biases between the results from the autoanalyzers and those from EIA. CONCLUSION: The two newly developed DCP assays showed high analytical performance, but re-establishment of reference limits would be necessary. The new analyzers could be useful for clinical laboratories because of convenience of operation and wide AMRs
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94557
DOI
10.1016/j.clinbiochem.2011.08.1144
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0009912011026324
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