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Evaluation of automated serum des-gamma-carboxyprothrombin (DCP) assays for detecting hepatocellular carcinoma

Authors
 Jonghyeon Choi  ;  Yongjung Park  ;  Jeong-Ho Kim  ;  Hyon-Suk Kim 
Citation
 CLINICAL BIOCHEMISTRY, Vol.44(17-18) : 1464-1468, 2011 
Journal Title
CLINICAL BIOCHEMISTRY
ISSN
 0009-9120 
Issue Date
2011
MeSH
Adult ; Aged ; Automation, Laboratory* ; Biomarkers/blood* ; Carcinoma, Hepatocellular/blood ; Carcinoma, Hepatocellular/diagnosis* ; Case-Control Studies ; Female ; Humans ; Immunoassay/methods ; Immunoassay/standards ; Liver Neoplasms/blood ; Liver Neoplasms/diagnosis* ; Male ; Middle Aged ; Protein Precursors/blood* ; Prothrombin ; Quality Control ; ROC Curve ; Reference Standards ; Reference Values
Keywords
Des-γ-carboxyprothrombin (DCP) ; Hepatocellular carcinoma (HCC) ; Protein induced by vitamin K absence or antagonist-II (PIVKA-II) ; Automated analyzer ; Reference range
Abstract
OBJECTIVES: We evaluated two new autoanalyzers, μTAS and Lumipulse for des-γ-carboxyprothrombin (DCP) assay.

MATERIAL AND METHODS: Analytical performance was evaluated, and the upper reference limit of the 97.5th percentile for DCP was re-established using sera from 140 healthy individuals. DCP levels were determined by the two autoanalyzers and EIA in a total of 239 sera from HCC patients (n=120) and those without HCC (n=119).

RESULTS: Total imprecision of the two automated assays was <5% CV. Analytical measurement ranges (AMRs) were verified to be linear. The new reference limits were 29.5 mAU/mL for μTAS and 35.0 mAU/mL for Lumipulse. There were proportional and constant biases between the results from the autoanalyzers and those from EIA.

CONCLUSION: The two newly developed DCP assays showed high analytical performance, but re-establishment of reference limits would be necessary. The new analyzers could be useful for clinical laboratories because of convenience of operation and wide AMRs
Full Text
http://www.sciencedirect.com/science/article/pii/S0009912011026324
DOI
10.1016/j.clinbiochem.2011.08.1144
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jeong Ho(김정호) ORCID logo https://orcid.org/0000-0003-2479-0548
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정) ORCID logo https://orcid.org/0000-0001-5668-4120
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94557
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