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Evaluation of a fully automated treponemal test and comparison with conventional VDRL and FTA-ABS tests.

Authors
 Yongjung Park  ;  Younhee Park  ;  Shin Young Joo  ;  Myoung Hee Park  ;  Hyon-Suk Kim 
Citation
 AMERICAN JOURNAL OF CLINICAL PATHOLOGY, Vol.136(5) : 705-710, 2011 
Journal Title
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
ISSN
 0002-9173 
Issue Date
2011
MeSH
Adult ; Aged ; Female ; Fluorescent Treponemal Antibody-Absorption Test/methods ; Humans ; Male ; Middle Aged ; Sensitivity and Specificity ; Syphilis/diagnosis* ; Syphilis Serodiagnosis/methods* ; Treponema Immobilization Test/methods ; Treponema pallidum/isolation & purification*
Keywords
Syphilis ; Treponemal test ; Architect Syphilis TP assay ; Venereal Disease Research Laboratory test ; VDRL test ; Fluorescent treponemal antibody absorption test ; FTA-ABS test
Abstract
We evaluated analytic performances of an automated treponemal test and compared this test with the Venereal Disease Research Laboratory test (VDRL) and fluorescent treponemal antibody absorption test (FTA-ABS). Precision performance of the Architect Syphilis TP assay (TP; Abbott Japan, Tokyo, Japan) was assessed, and 150 serum samples were assayed with the TP before and after heat inactivation to estimate the effect of heat inactivation. A total of 616 specimens were tested with the FTA-ABS and TP, and 400 were examined with the VDRL. The TP showed good precision performance with total imprecision of less than a 10% coefficient of variation. An excellent linear relationship between results before and after heat inactivation was observed (R(2) = 0.9961). The FTA-ABS and TP agreed well with a κ coefficient of 0.981. The concordance rate between the FTA-ABS and TP was the highest (99.0%), followed by the rates between FTA-ABS and VDRL (85.0%) and between TP and VDRL (83.8%). The automated TP assay may be adequate for screening for syphilis in a large volume of samples and can be an alternative to FTA-ABS.
Files in This Item:
T201103922.pdf Download
DOI
10.1309/AJCP0WOK0QDYGDCM
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정) ORCID logo https://orcid.org/0000-0001-5668-4120
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94556
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