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Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells

Authors
 Yun Soo Jang  ;  Kyoung-Ju Song  ;  Myeong Heon Shin  ;  Sang Kyou Lee  ;  Kyeong Ah Kim  ;  Young Ah Lee  ;  Ju Young Kim 
Citation
 Korean Journal of Parasitology, Vol.49(2) : 177-180, 2011 
Journal Title
 Korean Journal of Parasitology 
ISSN
 0023-4001 
Issue Date
2011
Abstract
Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94478
DOI
10.3347/kjp.2011.49.2.177
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실)
Yonsei Authors
김경아(Kim, Kyung Ah) ; 김주영(Kim, Ju Young) ; 송경주(Song, Kyoung Ju) ; 신명헌(Shin, Myeong Heon) ; 이영아(Lee, Young Ah)
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