Cited 5 times in
Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김경아 | - |
dc.contributor.author | 김주영 | - |
dc.contributor.author | 송경주 | - |
dc.contributor.author | 신명헌 | - |
dc.contributor.author | 이영아 | - |
dc.date.accessioned | 2014-12-20T17:22:22Z | - |
dc.date.available | 2014-12-20T17:22:22Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94478 | - |
dc.description.abstract | Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 177~180 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Calpain/antagonists & inhibitors | - |
dc.subject.MESH | Calpain/genetics | - |
dc.subject.MESH | Calpain/metabolism* | - |
dc.subject.MESH | Cell Death* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Dipeptides/metabolism | - |
dc.subject.MESH | Entamoeba histolytica/pathogenicity* | - |
dc.subject.MESH | Epithelial Cells/parasitology* | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | Humans | - |
dc.title | Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Yun Soo Jang | - |
dc.contributor.googleauthor | Kyoung-Ju Song | - |
dc.contributor.googleauthor | Ju Young Kim | - |
dc.contributor.googleauthor | Young Ah Lee | - |
dc.contributor.googleauthor | Kyeong Ah Kim | - |
dc.contributor.googleauthor | Sang Kyou Lee | - |
dc.contributor.googleauthor | Myeong Heon Shin | - |
dc.identifier.doi | 10.3347/kjp.2011.49.2.177 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02012 | - |
dc.contributor.localId | A02099 | - |
dc.contributor.localId | A02959 | - |
dc.contributor.localId | A00937 | - |
dc.contributor.localId | A00300 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 21738275 | - |
dc.subject.keyword | Entamoeba histolytica | - |
dc.subject.keyword | HT-29 colon epithelial cells | - |
dc.subject.keyword | calpain | - |
dc.subject.keyword | host cell death | - |
dc.contributor.alternativeName | Kim, Kyung Ah | - |
dc.contributor.alternativeName | Kim, Ju Young | - |
dc.contributor.alternativeName | Song, Kyoung Ju | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.alternativeName | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | Song, Kyoung Ju | - |
dc.contributor.affiliatedAuthor | Shin, Myeong Heon | - |
dc.contributor.affiliatedAuthor | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | Kim, Ju Young | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Ah | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 49 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 177 | - |
dc.citation.endPage | 180 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.49(2) : 177-180, 2011 | - |
dc.identifier.rimsid | 27357 | - |
dc.type.rims | ART | - |
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