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Luminex 단일항원검사법을 이용해 검출한 제공자 특이항체의 신장이식 후 항체매개성 거부반응 발생에 대한 예측도 분석

Other Titles
 Predictive Value of Donor Specific Antibody Measured by Luminex Single Antigen Assay for Antibody Mediated Rejection after Kidney Transplantation 
 주동진  ;  허규하  ;  김명수  ;  김현숙  ;  김순일  ;  김현정  ;  손승숙  ;  김혜진  ;  윤석준  ;  김유선 
 Journal of the Korean Society for Transplantation (대한이식학회지), Vol.25(3) : 169-175, 2011 
Journal Title
 Journal of the Korean Society for Transplantation  (대한이식학회지) 
Issue Date
Background: Luminex panel reactive antibody (PRA) is a method that is well known for its high sensitivity and specificity. By using a single antigen assay, the presence or absence of donor specific antibody (DSA) can be determined and its strength can be quantified in terms of the mean fluorescence intensity (MFI). In this study, we analyzed the correlation between the pre-transplant PRA and DSA measured by the Luminex method and the post-transplant clinical features after kidney transplantation. Methods: A total of 123 pre-transplant sera samples from kidney transplanted patients were tested. Luminex-PRA identification tests were performed using a Luminex fluoroanalyzer and a LifeCodes class I, II ID Kits. Single antigen assay by the Luminex method was used for detecting DSA and its MFI. Results: The positive Luminex-PRA group included more highly-sensitized patients such as women, patients with a previously positive lymphocyte cross match test and patients who were undergoing retransplantation. There was no correlation between the acute rejection rate and positive PRA on the Luminex-PRA. However, pretransplant DSA detected by the single antigen assay was significantly associated with episodes of antibody mediated rejection (P =0.047, OR=10.2), and DSA with higher MFI values (MFI≥3,000) was associated with antibody mediated rejection (P=0.023). Conclusions: Although pre-transplant positive PRA was not correlated with acute rejection episodes, the DSA measured by the Luminex single antigen assay seems to have a predictive value for post-transplant antibody mediated rejection
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실)
Yonsei Authors
김명수(Kim, Myoung Soo) ; 김순일(Kim, Soon Il) ; 김유선(Kim, Yu Seun) ; 김현숙(Kim, Hyon Suk) ; 주동진(Joo, Dong Jin) ; 허규하(Huh, Kyu Ha)
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