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Luminex 단일항원검사법을 이용해 검출한 제공자 특이항체의 신장이식 후 항체매개성 거부반응 발생에 대한 예측도 분석

Other Titles
 Predictive Value of Donor Specific Antibody Measured by Luminex Single Antigen Assay for Antibody Mediated Rejection after Kidney Transplantation 
Authors
 주동진  ;  허규하  ;  김유선  ;  윤석준  ;  김혜진  ;  손승숙  ;  김현정  ;  김순일  ;  김현숙  ;  김명수 
Citation
 Journal of the Korean Society for Transplantation, Vol.25(3) : 169-175, 2011 
Journal Title
Journal of the Korean Society for Transplantation(대한이식학회지)
ISSN
 1298-1711 
Issue Date
2011
Keywords
Histocompatibility antigen ; Kidney transplantation ; Rejection
Abstract
Background: Luminex panel reactive antibody (PRA) is a method that is well known for its high sensitivity and specificity.
By using a single antigen assay, the presence or absence of donor specific antibody (DSA) can be determined and its strength
can be quantified in terms of the mean fluorescence intensity (MFI). In this study, we analyzed the correlation between the
pre-transplant PRA and DSA measured by the Luminex method and the post-transplant clinical features after kidney
transplantation.
Methods: A total of 123 pre-transplant sera samples from kidney transplanted patients were tested. Luminex-PRA
identification tests were performed using a Luminex fluoroanalyzer and a LifeCodes class I, II ID Kits. Single antigen assay
by the Luminex method was used for detecting DSA and its MFI.
Results: The positive Luminex-PRA group included more highly-sensitized patients such as women, patients with a previously
positive lymphocyte cross match test and patients who were undergoing retransplantation. There was no correlation between
the acute rejection rate and positive PRA on the Luminex-PRA. However, pretransplant DSA detected by the single antigen
assay was significantly associated with episodes of antibody mediated rejection (P =0.047, OR=10.2), and DSA with higher
MFI values (MFI≥3,000) was associated with antibody mediated rejection (P=0.023).
Conclusions: Although pre-transplant positive PRA was not correlated with acute rejection episodes, the DSA measured by
the Luminex single antigen assay seems to have a predictive value for post-transplant antibody mediated rejection
Files in This Item:
T201103492.pdf Download
DOI
10.4285/jkstn.2011.25.3.169
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, Soon Il(김순일) ORCID logo https://orcid.org/0000-0002-0783-7538
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Joo, Dong Jin(주동진) ORCID logo https://orcid.org/0000-0001-8405-1531
Huh, Kyu Ha(허규하) ORCID logo https://orcid.org/0000-0003-1364-6989
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94398
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